Closed Alejandro1400 closed 3 months ago
To fulfill the feature requirements for single-frame localization of molecules using STORM techniques, the following steps were undertaken:
Loading the First Frame:
Threshold Normalization:
Pixel Resolution and Diffraction Limit:
Localization Process:
Gaussian Fitting:
Updating Maps:
The image below shows the result of the localization process. It displays the detected molecules overlaid on the first frame with their estimated positions marked.
In this example, 105 molecules were found and localized based on the detection threshold, pixel resolution, and diffraction limit parameters set by the user.
The purpose of this feature is to enable single-frame localization of molecules using STORM (Stochastic Optical Reconstruction Microscopy) techniques, this works as a 'Test parameters' so that the parameter selection can be tuned before starting the video processing. By utilizing the first frame of the loaded image, a defined threshold for molecule detection, pixel resolution, and the diffraction limit, the system will localize all molecules present in the frame.
Feature Details
Input Parameters:
Functionality:
Loading the First Frame:
Threshold Definition:
Pixel Resolution:
Diffraction Limit:
Localization Process:
UI Components:
Detection Threshold Input:
Det. Threshold:
Pixel Resolution Input:
Pixel Res. (nm):
Diffract. Limit Input:
Diffract. Limit:
Example Usage:
Expected Output: