AlexsLemonade / alsf-scpca

Management and analysis tools for ALSF Single-cell Pediatric Cancer Atlas data.
BSD 3-Clause "New" or "Revised" License
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Modify index building workflows to include pre mRNA index #75

Closed allyhawkins closed 3 years ago

allyhawkins commented 3 years ago

In this PR, I am altering the current build-index.nf workflow to build salmon and kallisto indexes using both the spliced.txome.fa and the spliced_intron.txome.fa that were generated in #68. Additionally, I updated all of the index's to now use the ensembl-103 build and the salmon index to use the container with the most recent salmon release (also used in the alevin-quant/run-alevin.nf workflow).

One thing to note is that generating the index using the spliced_intron.txome.fa takes considerably more memory, and that has also now been adjusted so the workflow runs successfully.