Note that bulk data is no longer required for multiplexing!

[jashapiro]

Just noting that we will also want to update the docs to note that bulk sequencing is no longer required too.

Originally posted by @allyhawkins in https://github.com/AlexsLemonade/scpca-nf/pull/329#pullrequestreview-1444530920

[sjspielman]

This functionality is planned to be included in the next release, so let's get those docs updated. Context: https://github.com/AlexsLemonade/scpca-nf/issues/353

Note that I've added this to our cite-seq epic; even though it isn't cite-seq specifically, it needs to happen under the overall scope of that epic given the planned release.

[jashapiro]

It is actually in the current release! That PR went straight to main.

[sjspielman]

It is actually in the current release! That PR went straight to main.

Ah well even more of a reason to do the thing!

[sjspielman]

Hm, I actually wonder if this issue should be in scpca-nf?

See here: https://github.com/AlexsLemonade/scpca-nf/blob/c9daa387f7e5137fe0bfde62bbe43de8d1a5ce5c/external-instructions.md?plain=1#L363-L365

[jashapiro]

Hm, I actually wonder if this issue should be in scpca-nf?

See here: https://github.com/AlexsLemonade/scpca-nf/blob/c9daa387f7e5137fe0bfde62bbe43de8d1a5ce5c/external-instructions.md?plain=1#L363-L365

That is updated and correct. We do need bulk for genetic demultiplexing, but not for cellhash demultiplexing, as the next sentence states.

[sjspielman]

Looking through backlog issues, and I think this can be closed? In processing_information.md, bulk re-multiplexing is only mentioned for genetic, not HTO.

[jashapiro]

Looks good to me. Closing