Get screen calibration (gamma, luminance) to Pamela from MPE

[jeromelecoq]

Pamela would like to know more details on gamma and luminance calibration on the 2p rigs

[jeromelecoq]

I asked MPE today and we are waiting for their answer: Here is my email: Hi Colin and Alex,

One of the OpenScope 2022 team was curious about our screen calibration data for the neuronal recording rig. This is because they want to compare their stimuli condition with existing published datasets.

Do you have access to some of this data that we could share? I think they are interested in luminance and perhaps the linearization that is applied to correct for gamma.

Thanks! Jerome

[jeromelecoq]

This, I am not sure, I will let Colin and Alex comment.

From: Pamela Reinagel [pamela.reinagel@gmail.com](mailto:pamela.reinagel@gmail.com) Date: Monday, July 10, 2023 at 5:44 PM To: Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org) Cc: reinagelucsd [reinagelucsd@gmail.com](mailto:reinagelucsd@gmail.com), Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org), Alex Cahoon [alex.cahoon@alleninstitute.org](mailto:alex.cahoon@alleninstitute.org), Severine Durand [severined@alleninstitute.org](mailto:severined@alleninstitute.org) Subject: Re: Gamma and luminance calibration on openscope neuropixel rig

CAUTION: This email originated from outside the Allen Institute. Please do not click links or open attachments unless you've validated the sender and know the content is safe. OK, the calibration file says "GammaLuminance.37" I was wondering if it should be "GammaLuminance.50" to go with monitor_name: "Gamma1.Luminance50"

On Mon, Jul 10, 2023 at 4:51 PM Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org) wrote: It should be, this is how we should be requesting the calibration on the rig.

From: Pamela Reinagel [pamela.reinagel@gmail.com](mailto:pamela.reinagel@gmail.com) Date: Monday, July 10, 2023 at 11:54 AM To: reinagelucsd [reinagelucsd@gmail.com](mailto:reinagelucsd@gmail.com) Cc: Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org), Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org), Alex Cahoon [alex.cahoon@alleninstitute.org](mailto:alex.cahoon@alleninstitute.org), Severine Durand [severined@alleninstitute.org](mailto:severined@alleninstitute.org) Subject: Re: Gamma and luminance calibration on openscope neuropixel rig

CAUTION: This email originated from outside the Allen Institute. Please do not click links or open attachments unless you've validated the sender and know the content is safe. I notice that in the "regimen" I was sent it says monitor_name: "Gamma1.Luminance50"

is this consistent with the calibration data I just saw?

P.

On Mon, Jul 10, 2023 at 10:50 AM Pamela Reinagel at UCSD [reinagelucsd@gmail.com](mailto:reinagelucsd@gmail.com) wrote: but I do think this information needs to be findable

Sent from my iPhone

On Jul 10, 2023, at 10:34 AM, Pamela Reinagel at UCSD [reinagelucsd@gmail.com](mailto:reinagelucsd@gmail.com) wrote:

 OK got it. The linearization looks great, the color balance doesn't really matter, the only piece of information I need to know is:

Optimal Brightness cd/m^2: 37

I just want to confirm what "optimal brightness" means, is this the max luminance when all three guns are on at 100%? Corresponding to ~14200 counts in the bottom right graph?

Thanks P.

On Mon, Jul 10, 2023 at 9:56 AM Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org) wrote: I’ve attached the report.

Regards, Colin.

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From: Pamela Reinagel at UCSD [reinagelucsd@gmail.com](mailto:reinagelucsd@gmail.com) Sent: Monday, July 10, 2023 8:51 AM To: Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org) Cc: Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org); Alex Cahoon [alex.cahoon@alleninstitute.org](mailto:alex.cahoon@alleninstitute.org); Pamela Reinagel [pamela.reinagel@gmail.com](mailto:pamela.reinagel@gmail.com); Severine Durand [severined@alleninstitute.org](mailto:severined@alleninstitute.org) Subject: Re: Gamma and luminance calibration on openscope neuropixel rig

CAUTION: This email originated from outside the Allen Institute. Please do not click links or open attachments unless you've validated the sender and know the content is safe. I don’t think you attached the report

Sent from my iPhone

On Jul 10, 2023, at 12:57 AM, Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org) wrote:



Hi Pamela Colin and Alex, cced, are managing the screen calibration process as you can see below

Could you give us a little more details as to what you need for understanding the stimuli ?

Thanks

Get Outlook for iOS From: Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org) Sent: Wednesday, July 5, 2023 8:06:50 PM To: Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org); Alex Cahoon [alex.cahoon@alleninstitute.org](mailto:alex.cahoon@alleninstitute.org) Cc: Severine Durand [severined@alleninstitute.org](mailto:severined@alleninstitute.org) Subject: RE: Gamma and luminance calibration on openscope neuropixel rig

Hi Jerome,

I’ve attached an example the report we generate after calibration of both luminance and gamma correction (the reports are all the same, this example came off one of the behavior rigs). If feel like sharing this will likely result in more questions; would you like me to get the information on the hardware we use for the calibrations as well?

Regards, Colin.

Get Help from Engineering: View/Submit Tickets / Visit Instrumentation Resource Site

From: Jerome Lecoq [jeromel@alleninstitute.org](mailto:jeromel@alleninstitute.org) Sent: Monday, July 3, 2023 4:29 PM To: Colin Farrell [colinf@alleninstitute.org](mailto:colinf@alleninstitute.org); Alex Cahoon [alex.cahoon@alleninstitute.org](mailto:alex.cahoon@alleninstitute.org) Cc: Severine Durand [severined@alleninstitute.org](mailto:severined@alleninstitute.org) Subject: Gamma and luminance calibration on openscope neuropixel rig

Hi Colin and Alex,

One of the OpenScope 2022 team was curious about our screen calibration data for the neuronal recording rig. This is because they want to compare their stimuli condition with existing published datasets.

Do you have access to some of this data that we could share? I think they are interested in luminance and perhaps the linearization that is applied to correct for gamma.

Thanks!

Jerome

-- Pamela Reinagel Department of Neurobiology School of Biological Sciences University of California, San Diego 9500 Gilman Drive #0357 La Jolla, CA 92093 www.reinagellab.org

[jeromelecoq]

Go back to Pamela on this