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AlsammanAlsamman / KASPspoon

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Primers cannot have IUPAC ambiguities #1

Open cbird808 opened 5 months ago

cbird808 commented 5 months ago

I tried this out, but the in silico pcr would only return results if there were no IUPAC ambiguities in the primers. Otherwise, it works quite nicely

AlsammanAlsamman commented 5 months ago

The code was written a long time ago. Could you please provide me with a sample input?

cbird808 commented 5 months ago

Here's a degenerate primer set and some templates

From: AlsammanAlsamman @.> Sent: Thursday, February 8, 2024 4:08 PM To: AlsammanAlsamman/KASPspoon @.> Cc: Bird, Chris @.>; Author @.> Subject: Re: [AlsammanAlsamman/KASPspoon] Primers cannot have IUPAC ambiguities (Issue #1)

The code was written a long time ago. Could you please provide me with a sample input?

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AlsammanAlsamman commented 5 months ago

I can not find it, please send by email smahmoud[at]ageri.sci.eg