Is it possible to do the METABOLIC_C with the contigs not the genomes?

[Sharifah89]

Hello, Is it possible to do the METABOLIC_C with the contigs not the genomes? Instead of using the genomes I add the contigs FASTA files.

[ChaoLab]

Since GTDB-Tk was used to assign each input genome a taxonomy, if you use contigs instead of genomes, GTDB-Tk will treat each contig as a genome, your genome results will be based on very fragmented genomes. The taxonomical results should not be reliable, and moreover, the downstream diagrams using the taxonomical result will be somehow meaningless

[Sharifah89]

So the METABOLIC_G can work to make the functional profile?

[ChaoLab]

yes