Metagenome sample numbering

[B-1991-ing]

Hi Zhichao,

The screenshot are my 6 metagenome fastq files, will the METABOLIC software recognise my "Sample2" as file1, "Sample3" as file2, "Sample4" as file3, "Sample5" as file4, "Sample6" as file5, "Sample7" as file6?

Best,

Bing

[ChaoLab]

No, METABOLIC will combined all the fastq files. If you want to get mapping results and subsequent networks, MW scores, etc, you will need to run them separately

[B-1991-ing]

Hi Zhichao,

The problem is that I put the my 55 MAGs and 6 metagenomes (Sample2", "Sample3", "Sample4", "Sample5", "Sample6", "Sample7") as the input of the METABOLIC. But, in the METABOLIC output, it gives me "Sample1", "Sample2", "Sample3", "Sample4", "Sample5", "Sample6". For example, All_gene_collections_mapped.1.sorted.bam, All_gene_collections_mapped.2.sorted.bam, All_gene_collections_mapped.3.sorted.bam, All_gene_collections_mapped.4.sorted.bam, All_gene_collections_mapped.5.sorted.bam, All_gene_collections_mapped.6.sorted.bam. All_gene_collections_mapped.depth copy.txt

So, can I think as below? All_gene_collections_mapped.1.sorted.bam - my Sample2 All_gene_collections_mapped.2.sorted.bam- my Sample3 All_gene_collections_mapped.3.sorted.bam- my Sample4 All_gene_collections_mapped.4.sorted.bam- my Sample5 All_gene_collections_mapped.5.sorted.bam- my Sample6 All_gene_collections_mapped.6.sorted.bam- my Sample7

Bing

[ChaoLab]

Yes, the order is based on the raw numbers in your inputted read txt file. If Sample 2, 3, 4 ... 7 is the order of fastq files you placed, then yes to the corresponding relationship

[B-1991-ing]

Thank you very much for your reply, Zhichao.