Arcadia-Science / noveltree

NovelTree is a highly parallelized and computationally efficient phylogenomic workflow that infers gene families, gene family trees, species trees, and gene family evolutionary history.
GNU Affero General Public License v3.0
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Alignment process is failing #102

Open mliannholland opened 2 months ago

mliannholland commented 2 months ago

Description of the bug

The cogeqc process now works but now the pipeline is stuck on the alignment processes

Command used and terminal output

executor >  local (182)
[22/cb320f] process > NOVELTREE:INPUT_CHECK:SAMPLESHEET_CHECK (tsar_downsamp_test_samplesheet.csv) [100%] 1 of 1 ✔
[ec/9adfba] process > NOVELTREE:ANNOTATE_UNIPROT (Tetrahymena_thermophila)                         [100%] 5 of 5 ✔
executor >  local (182)
[22/cb320f] process > NOVELTREE:INPUT_CHECK:SAMPLESHEET_CHECK (tsar_downsamp_test_samplesheet.csv) [100%] 1 of 1 ✔
[ec/9adfba] process > NOVELTREE:ANNOTATE_UNIPROT (Tetrahymena_thermophila)                         [100%] 5 of 5 ✔
[5f/fd9563] process > NOVELTREE:ORTHOFINDER_PREP_TEST (Prepping data for OrthoFinder)              [100%] 1 of 1 ✔
[4b/9e68e4] process > NOVELTREE:DIAMOND_BLASTP_TEST (Telonema_subtile)                             [100%] 36 of 36 ✔
[9e/461923] process > NOVELTREE:ORTHOFINDER_MCL_TEST (MCL clustering)                              [100%] 5 of 5 ✔
[e6/f94536] process > NOVELTREE:COGEQC (Orthogroup Summary)                                        [100%] 5 of 5 ✔
[a7/4bc595] process > NOVELTREE:SELECT_INFLATION (Selecting best MCL Inflation)                    [100%] 1 of 1 ✔
[17/657760] process > NOVELTREE:BUSCO_SHALLOW (Telonema_sp_P-2)                                    [100%] 7 of 7 ✔
[a5/de6b4f] process > NOVELTREE:BUSCO_BROAD (Telonema_sp_P-2)                                      [100%] 7 of 7 ✔
[7e/10067b] process > NOVELTREE:ORTHOFINDER_PREP_ALL (Prepping data for OrthoFinder)               [100%] 1 of 1 ✔
[7f/1f28fa] process > NOVELTREE:DIAMOND_BLASTP_ALL (Tetrahymena_thermophila)                       [100%] 49 of 49 ✔
[7b/88b6ea] process > NOVELTREE:ORTHOFINDER_MCL_ALL (MCL clustering)                               [100%] 1 of 1 ✔
[26/00c80f] process > NOVELTREE:FILTER_ORTHOGROUPS (Summarize OG taxon distribution)               [100%] 1 of 1 ✔
[8f/c935d8] process > NOVELTREE:ALIGN_SEQS (OG0000034)                                             [ 50%] 24 of 48, failed: 24, retries: 24
[b1/206451] process > NOVELTREE:ALIGN_REMAINING_SEQS (OG0000174)                                   [ 49%] 37 of 75, failed: 37, retries: 37
[-        ] process > NOVELTREE:INFER_TREES                                                        -
[-        ] process > NOVELTREE:INFER_REMAINING_TREES                                              -
[-        ] process > NOVELTREE:ASTEROID                                                           -
[-        ] process > NOVELTREE:SPECIESRAX                                                         -
[-        ] process > NOVELTREE:GENERAX_PER_FAMILY                                                 -
[-        ] process > NOVELTREE:GENERAX_PER_SPECIES                                                -
[-        ] process > NOVELTREE:ORTHOFINDER_PHYLOHOGS                                              -
[bc/d858ca] NOTE: Process `NOVELTREE:ALIGN_SEQS (OG0000088)` terminated with an error exit status (125) -- Execution is retried (1)
[8f/c935d8] NOTE: Process `NOVELTREE:ALIGN_SEQS (OG0000034)` terminated with an error exit status (125) -- Execution is retried (1)
[b5/70311f] NOTE: Process `NOVELTREE:ALIGN_REMAINING_SEQS (OG0000114)` terminated with an error exit status (125) -- Execution is retried (1)
[bc/7e0a78] NOTE: Process `NOVELTREE:ALIGN_REMAINING_SEQS (OG0000108)` terminated with an error exit status (125) -- Execution is retried (1)
[66/11981d] NOTE: Process `NOVELTREE:ALIGN_REMAINING_SEQS (OG0000162)` terminated with an error exit status (125) -- Execution is retried (1)

Relevant files

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System information

aws ec2 docker linux latest version from this week v1.0.2