Error in `dplyr::bind_rows()`: Cannot Combine Different Data Types - Seeking Assistance from CircRNAprofiler Author

[Li-Yaoting]

Hello, Authors of CircRNAprofiler. I'm encountering an error when using the module backSplicedJunctions <- getBackSplicedJunctions(gtf). The error message says " Error in dplyr::bind_rows(): ! Can't combine ..1$startUpBSE and ..2$startUpBSE . Could you provide guidance on how to resolve this issue? I am using the following package versions: R (v 4.3.1), CircRNAprofiler (v 1.14), and dplyr (v 1.1.2). The data I am using is the output after annotation of circExplorer2 (v2.3.8), with the addition of a header before reading. The data format is shown in the image attached. Prior to analysis with CircRNAprofiler, I checked the project folder using checkProjectFolder, and the result was 0. Could you please advise on how to address this dplyr data merging error? Thank you.

[Li-Yaoting]

environment and package version

[Aufiero]

Sorry for the late reply. I´ll have a look and come back to you in the following days.

[Aufiero]

If you send me the file I will do a test and let you know.

[Li-Yaoting]

The following file is a test file that can be used, exported from the circExplorer2 software. SRR6890663_GSM3066231_G01S01Y_Young_1.known.txt

[Aufiero]

circRNAprofiler was tested with circExplorer2 v2.3.4 output file (circularRNA_full.txt). I tested with the latter file and it was working fine. The error arise when I used the file that you have provided. In any case I fixed the error dplyr::bind_rows(), so you should now be able to use it. Install the latest version ( Release 3.18) of circRNaprofiler.

[Aufiero]

Could you let me know if it works?

[Li-Yaoting]

Thanks,it work. However, if the first line of the circularRNA_full.txt file has "ciRNA" as circtype, an error will occur. I hope it can be fixed, corresponding to the circularRNA_full.txt file. SRR20204938_GSM6338657_29256.2.circularRNA_known.txt

[Aufiero]

Why do you have ciRNA rather than circRNA?

[Aufiero]

What you can do for now is to preprocess the file, by changing ciRNA with circRNA and it should work.