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BD2KGenomics
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toil-rnaseq
UC Santa Cruz Computational Genomics Lab's Toil-based RNA-seq pipeline
Apache License 2.0
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Add BAM umend QC
#158
Closed
jvivian
closed
6 years ago
jvivian
commented
6 years ago
Input
STAR BAM
Output
readDist.txt: The output of RSeqQC read_distribution.py (~1kb)
bam_umend_qc.tsv: uniqMappedNonDupeReadCount, estExonicUniqMappedNonDupeReadCount and PASS/FAIL
bam_umend_qc.json: Same as bam_umend_qc.tsv but in json format
sortedByCoord.md.bam: BAM with duplicates marked sorted by coordinate
sortedByCoord.md.bam.bai: Index for sortedByCoord.md.bam
Command
docker run --rm \ -v
:/inputs/sample.bam \ -v
:/outputs \ -v
:/tmp \ ucsctreehouse/bam-umend-qc \ /inputs/sample.bam \ /outputs
https://github.com/UCSC-Treehouse/bam-umend-qc
https://github.com/UCSC-Treehouse/bam-umend-qc