stereo-seq怎么对接Velocity

[liyu-ynu]

你好，请问一下，我用SAW标准流程02.count这一步得到的bam文件，用Velocity run准备把bam转换为loom，出现了以下错误，想问一下是什么原因？感谢您解答，谢谢。 Traceback (most recent call last): File "/public/home/liyu/miniconda3/envs/r4.2/bin/velocyto", line 11, in sys.exit(cli()) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/click/core.py", line 1157, in call return self.main(args, kwargs) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/click/core.py", line 1078, in main rv = self.invoke(ctx) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/click/core.py", line 1688, in invoke return _process_result(sub_ctx.command.invoke(sub_ctx)) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/click/core.py", line 1434, in invoke return ctx.invoke(self.callback, ctx.params) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/click/core.py", line 783, in invoke return __callback(args, **kwargs) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/velocyto/commands/run.py", line 113, in run return _run(bamfile=bamfile, gtffile=gtffile, bcfile=bcfile, outputfolder=outputfolder, File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/velocyto/commands/_run.py", line 159, in _run exincounter.peek(bamfile[0]) File "/public/home/liyu/miniconda3/envs/r4.2/lib/python3.10/site-packages/velocyto/counter.py", line 158, in peek raise IOError("The bam file does not contain cell and umi barcodes appropriatelly formatted. If you are runnin UMI-less data you should use the -U flag.") OSError: The bam file does not contain cell and umi barcodes appropriatelly formatted. If you are runnin UMI-less data you should use the -U flag.