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BICCN / TMN

Repository for work on neuroscience techniques ontology development.
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Technique for Iso-seq / ScISOr-seq #73

Closed kasbaker closed 2 years ago

kasbaker commented 2 years ago

Preferred term label

Iso-seq or ScISOr-seq

Synonyms

PacBio Iso-seq

Textual definition

https://www.pacb.com/blog/introduction-of-the-iso-seq-method-state-of-the-art-for-full-length-transcriptome-sequencing/#:~:text=The%20Iso%2DSeq%20method%20produces,for%20transcript%20assembly%20or%20inferencing.

https://pubmed.ncbi.nlm.nih.gov/30320766/

Suggested parent term

BAO:0003027

BAO:0040026

https://www.ebi.ac.uk/ols/ontologies/bao/terms?iri=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23BAO_0003027&viewMode=All&siblings=false

Sequencing is actually DNA sequencing, but it is cDNA replicated based on the RNA in the cell's gene expression.

Comments

There is some politics involved about whether to call this Iso-seq or SciSOr-seq. PacBio has a commercial protocol available for this technique.

patrick-lloyd-ray commented 2 years ago

Thanks, @kasbaker! (DT-3565)