BIMSBbioinfo / pigx_rnaseq

Bulk RNA-seq Data Processing, Quality Control, and Downstream Analysis Pipeline
GNU General Public License v3.0
20 stars 11 forks source link

Changing the cDNA fasta should not trigger rerun of hisat2 branch #142

Open alexg9010 opened 2 months ago

alexg9010 commented 2 months ago

After finishing a pipeline run I wanted to change the cDNA file to trigger a rerun of salmon, but this also triggered a restart of Hisat because of the rule check_annotation_files. Given that the cDNA is not relevant for Hisat2 mapping I propose a more fine-grained solution that separately checks GTF vs DNA and GTF vs cDNA.

For now, I can circumvent the rerun by using:

 pigx-rnaseq -s settings_editted.yaml sample_sheet_merged_replicates.csv --target={deseq_report_salmon_transcripts,deseq_report_salmon_genes}