BSSeeker / BSseeker2

A versatile aligning pipeline for bisulfite sequencing data
http://pellegrini.mcdb.ucla.edu/BS_Seeker2/
MIT License
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Pysam 0.11 align error #50

Open desmodus1984 opened 7 months ago

desmodus1984 commented 7 months ago

Hi,

I created a new conda environment, I installed samtools 1.19 for merging and sorting, and then installed bs-seeker2. When I tried doing the alignment I got the error:

[Error] It seems that you haven't install "pysam" package.. Please do it before you run this script.

In my environment pysam is installed but Bs-seeker2 is not detecting it. pysam 0.11.2.2 py27_1 bioconda

I had the same problem, created a brand new environment with a brand new installed, and Bs-seeker is failing. I did installed it in my lab server and it worked ,but the alignment is very slow, but installing in the HPC failed. How can I fix this?

Thanks