When I'm using Clust with multiple datasets I get a warning message during the "Cluster optimization and completion" step. The output seems to be alright, except that I get no PDF with the cluster plots.
(clust) apcamargo@elementaryos:~/clust$ python clust.py ExampleData/1_RawData/Data/
/===========================================================================\
| Clust |
| (Optimised consensus clustering of multiple heterogenous datasets) |
| Python package version 1.8.10 (2018) Basel Abu-Jamous |
+---------------------------------------------------------------------------+
| Analysis started at: Monday 26 November 2018 (17:27:17) |
| 1. Reading dataset(s) |
| 2. Data pre-processing |
| - Automatic normalisation mode (default in v1.7.0+). |
| Clust automatically normalises your dataset(s). |
| To switch it off, use the `-n 0` option (not recommended). |
| Check https://github.com/BaselAbujamous/clust for details. |
| - Flat expression profiles filtered out (default in v1.7.0+). |
| To switch it off, use the --no-fil-flat option (not recommended). |
| Check https://github.com/BaselAbujamous/clust for details. |
| 3. Seed clusters production (the Bi-CoPaM method) |
| 10% |
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| 4. Cluster evaluation and selection (the M-N scatter plots technique) |
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| 5. Cluster optimisation and completion |
/home/apcamargo/anaconda3/envs/clust/lib/python2.7/site-packages/numpy/core/fromnumeric.py:2920: RuntimeWarning: Mean of empty slice.
out=out, **kwargs)
| 6. Saving results in |
| /home/apcamargo/clust/Results_26_Nov_18 |
| Error: could not save clusters plots in a PDF file. |
| Resuming producing the other results files ... |
+---------------------------------------------------------------------------+
| Analysis finished at: Monday 26 November 2018 (17:28:09) |
| Total time consumed: 0 hours, 0 minutes, and 52 seconds |
| |
\===========================================================================/
/===========================================================================\
| RESULTS SUMMARY |
+---------------------------------------------------------------------------+
| Clust received 3 datasets with 9332 unique genes. After filtering, 9330 |
| genes made it to the clustering step. Clust generated 14 clusters of |
| genes, which in total include 4686 genes. The smallest cluster includes |
| 27 genes, the largest cluster includes 1231 genes, and the average |
| cluster size is 334.714285714 genes. |
+---------------------------------------------------------------------------+
| Citation |
| ~~~~~~~~ |
| When publishing work that uses Clust, please include this citation: |
| Basel Abu-Jamous and Steven Kelly (2018) Clust: automatic extraction of |
| optimal co-expressed gene clusters from gene expression data. Genome |
| Biology 19:172; doi: https://doi.org/10.1186/s13059-018-1536-8. |
+---------------------------------------------------------------------------+
| For enquiries contact: |
| Basel Abu-Jamous |
| Department of Plant Sciences, University of Oxford |
| basel.abujamous@plants.ox.ac.uk |
| baselabujamous@gmail.com |
\===========================================================================/
Hi Basel,
When I'm using Clust with multiple datasets I get a warning message during the "Cluster optimization and completion" step. The output seems to be alright, except that I get no PDF with the cluster plots.