BenLangmead / bowtie

An ultrafast memory-efficient short read aligner
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bowtie alignmnet produces sam bam files sizes for different fastq files #140

Open deepthi-omics opened 1 month ago

deepthi-omics commented 1 month ago

~/bowtie-1.3.1-linux-x86_64 This version of bowtie in HPC produces sam or bam files even with different fastq files and gives Error reading _rstarts[] array: 21720, 23124 I have pasted my code below for reference and aslo error.Thanks.

./bowtie --no-unal --threads 3 --sam ~/neural_GSE181050/indexes/GRCh38 ~/hspiRNA_13690/cut_results/adult_cbl_ERR12409218t.fastq ~/hspiRNA_13690/bam_files/adult_cbl_ERR12409218t.bam Setting the index via positional argument will be deprecated in a future release. Please use -x option instead. Error reading _rstarts[] array: 21720, 23124