Number of bins prior to reclustering: 0

[yazhinia]

Hi, Semibin2 shows problem with producing bins SemiBin[86060] INFO Number of bins prior to reclustering: 0. Due to this, the following error occurs with Sequence file /semibin_tmp/tmpcyhmrovl/orfs.faa is empty or misformatted even though input is correct (sorted bam files and fasta contig file).

I also see problem with SemiBin[158473] INFO Did not detect GPU, using CPU. I run the script on gpu node but still it didn't detect and therefore very slow util it reaches the above error.

Any help in solving is much appreciated.

[hiroakif93]

The same problem happened to me when I use SemiBin2. BINNING.log

Are there any updates?

Below is the mycode

SemiBin2 single_easy_bin \
--self-supervised \
-i contig.fasta \
-b mapped.sorted.bam \
-o output --tmpdir tmpdir \
--threads 2 --engine gpu --random-seed 222 --compression gz

Thanks in advance.