celiosantosjr
commented
1 year ago Dear aslangabriel99,
I am sorry to hear that. However, 16S amplicon is not an appropriate input for Macrel. It uses metagenomic datasets, therefore, it is expected 16S data do not return any result. For the metagenomes sequenced from environmental sources, one possible option is trying to use the flag '--keep-negatives' to verify if any peptide was spotted. The input influences heavily the results in this case, and as Macrel is a high precision method, its recall is not as extensive as in other lower precision methods. If you are willing to try, please let me know what the results were. Besides, for questions and other discussions like this one, you can always go to our discussion forum.
Best, Celio
On Fri, May 19, 2023 at 9:26 AM aslangabriel99 @.***> wrote:
Hi, I have tested the gut 16s amplicon of a lot of host such as duck, blacksoldierfly,silkworm, housefly, et .al and some enviromental metageomes sequnced on illumina by paire ends. Unfortunately there is no result in prediction gz files. I also tested with the example data. The result is normal. I don't know what's the problem
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Hi, I have tested the gut 16s amplicon of a lot of host such as duck, blacksoldierfly,silkworm, housefly, et .al and some enviromental metageomes sequnced on illumina by paire ends. Unfortunately there is no result in prediction gz files. I also tested with the example data. The result is normal. I don't know what's the problem