vbgmm import error

[gwendolin]

Hi,

I saw a couple similar issues to this one on the forum, but I haven't been able to fix it myself. I am getting this error when trying to run concoct -h:

(concoct_env) science@SC-Bioinformatics:~$ concoct -h Traceback (most recent call last): File "/home/science/miniconda3/envs/concoct_env/bin/concoct", line 6, in import vbgmm ImportError: libmkl_rt.so: cannot open shared object file: No such file or directory

I downloaded the latest version of concoct with conda as described in the installation docs. My operating system is Linux Mint v20 (Ulyana) And this is what I get with conda list:

(concoct_env) science@SC-Bioinformatics:~$ conda list

packages in environment at /home/science/miniconda3/envs/concoct_env:

#

Name Version Build Channel

_libgcc_mutex 0.1 conda_forge conda-forge _openmp_mutex 4.5 1_gnu conda-forge biopython 1.78 py38h1e0a361_1 conda-forge blas 1.0 mkl
bzip2 1.0.8 h516909a_3 conda-forge c-ares 1.16.1 h516909a_3 conda-forge ca-certificates 2020.6.20 hecda079_0 conda-forge certifi 2020.6.20 py38h924ce5b_2 conda-forge concoct 1.1.0 py38h7be5676_2 bioconda cython 0.29.21 py38h348cfbe_1 conda-forge gsl 2.6 hf94e986_0 conda-forge htslib 1.11 hd3b49d5_0 bioconda joblib 0.17.0 py_0 conda-forge krb5 1.17.1 hfafb76e_3 conda-forge ld_impl_linux-64 2.35 h769bd43_9 conda-forge libblas 3.9.0 1_h86c2bf4_netlib conda-forge libcblas 3.9.0 2_h86c2bf4_netlib conda-forge libcurl 7.71.1 hcdd3856_8 conda-forge libdeflate 1.6 h516909a_0 conda-forge libedit 3.1.20191231 he28a2e2_2 conda-forge libev 4.33 h516909a_1 conda-forge libffi 3.2.1 he1b5a44_1007 conda-forge libgcc-ng 9.3.0 h5dbcf3e_17 conda-forge libgfortran-ng 9.3.0 he4bcb1c_17 conda-forge libgfortran5 9.3.0 he4bcb1c_17 conda-forge libgomp 9.3.0 h5dbcf3e_17 conda-forge liblapack 3.9.0 2_h86c2bf4_netlib conda-forge libnghttp2 1.41.0 h8cfc5f6_2 conda-forge libssh2 1.9.0 hab1572f_5 conda-forge libstdcxx-ng 9.3.0 h2ae2ef3_17 conda-forge llvm-openmp 8.0.1 hc9558a2_0 conda-forge ncurses 6.2 he1b5a44_2 conda-forge nose 1.3.7 py_1006 conda-forge numpy 1.19.2 py38hf89b668_1 conda-forge openmp 8.0.1 0 conda-forge openssl 1.1.1h h516909a_0 conda-forge pandas 1.1.4 py38h0ef3d22_0 conda-forge pip 20.2.4 py_0 conda-forge python 3.8.6 h852b56e_0_cpython conda-forge python-dateutil 2.8.1 py_0 conda-forge python_abi 3.8 1_cp38 conda-forge pytz 2020.4 pyhd8ed1ab_0 conda-forge readline 8.0 he28a2e2_2 conda-forge samtools 1.11 h6270b1f_0 bioconda scikit-learn 0.23.2 py38h5d63f67_2 conda-forge scipy 1.5.3 py38hb2138dd_0 conda-forge setuptools 49.6.0 py38h924ce5b_2 conda-forge six 1.15.0 pyh9f0ad1d_0 conda-forge sqlite 3.33.0 h4cf870e_1 conda-forge threadpoolctl 2.1.0 pyh5ca1d4c_0 conda-forge tk 8.6.10 hed695b0_1 conda-forge wheel 0.35.1 pyh9f0ad1d_0 conda-forge xz 5.2.5 h516909a_1 conda-forge zlib 1.2.11 h516909a_1010 conda-forge ` Any help would be greatly appreciated! Tagging my colleague for reference: @DavidGeeraerts

[DavidGeeraerts]

(Cc @gwendolin )

Is it odd that the libmkl_rt.so is pointing to a file path that doesn't exist? ["filepath": "/home/ogrisel/miniconda3/envs/tmp/lib/libmkl_rt.so"]

Seems like this might be the issue?

This might explain why concoct --help doesn't even work?

[cwood15]

Hello,

I seem to be having the same issue. When I try to run concoct I get the error: Traceback (most recent call last): File "/home/cwood15/anaconda3/envs/concoct_env/bin/concoct", line 6, in import vbgmm ImportError: libmkl_rt.so: cannot open shared object file: No such file or directory

My input was: concoct --composition_file contigs_10K.fasta --coverage_file coverage_table.tsv -b concoct_output

I installed concoct using the recommended bioconda installation on https://concoct.readthedocs.io/en/latest/installation.html

Here is my conda environment

packages in environment at /home/cwood15/anaconda3/envs/concoct_env: Name Version Build Channel _libgcc_mutex 0.1 main biopython 1.78 py38h1e0a361_1 conda-forge blas 1.0 mkl bzip2 1.0.8 h516909a_3 conda-forge c-ares 1.11.0 h470a237_1 bioconda ca-certificates 2020.6.20 hecda079_0 conda-forge certifi 2020.6.20 py38h924ce5b_2 conda-forge concoct 1.1.0 py38h7be5676_2 bioconda cython 0.29.21 py38h348cfbe_1 conda-forge gsl 2.6 hf94e986_0 conda-forge htslib 1.11 hd3b49d5_0 bioconda joblib 0.17.0 py_0 conda-forge krb5 1.17.1 hfafb76e_3 conda-forge ld_impl_linux-64 2.33.1 h53a641e_7 libblas 3.9.0 1_h6e990d7_netlib conda-forge libcblas 3.9.0 2_h6e990d7_netlib conda-forge libcurl 7.71.1 hcdd3856_8 conda-forge libdeflate 1.6 h516909a_0 conda-forge libedit 3.1.20191231 h14c3975_1 libev 4.33 h516909a_1 conda-forge libffi 3.3 he6710b0_2 libgcc-ng 9.1.0 hdf63c60_0 libgfortran-ng 7.5.0 hae1eefd_17 conda-forge libgfortran4 7.5.0 hae1eefd_17 conda-forge liblapack 3.9.0 2_h6e990d7_netlib conda-forge libnghttp2 1.41.0 hab1572f_1 conda-forge libssh2 1.9.0 hab1572f_5 conda-forge libstdcxx-ng 9.1.0 hdf63c60_0 llvm-openmp 8.0.1 hc9558a2_0 conda-forge ncurses 6.2 he6710b0_1 nose 1.3.7 py_1006 conda-forge numpy 1.19.4 py38hf0fd68c_0 conda-forge openmp 8.0.1 0 conda-forge openssl 1.1.1h h516909a_0 conda-forge pandas 1.1.4 py38h0ef3d22_0 conda-forge pip 20.2.4 py_0 conda-forge python 3.8.5 h7579374_1 python-dateutil 2.8.1 py_0 conda-forge python_abi 3.8 1_cp38 conda-forge pytz 2020.4 pyhd8ed1ab_0 conda-forge readline 8.0 h7b6447c_0 samtools 1.11 h6270b1f_0 bioconda scikit-learn 0.23.2 py38h5d63f67_2 conda-forge scipy 1.5.3 py38h828c644_0 conda-forge setuptools 49.6.0 py38h924ce5b_2 conda-forge six 1.15.0 pyh9f0ad1d_0 conda-forge sqlite 3.33.0 h62c20be_0 threadpoolctl 2.1.0 pyh5ca1d4c_0 conda-forge tk 8.6.10 hbc83047_0 tzdata 2020d h14c3975_0 wheel 0.35.1 py_0 xz 5.2.5 h7b6447c_0 zlib 1.2.11 h7b6447c_3

Any help would be greatly appreciated! Thank you!

Claudia

[DavidGeeraerts]

@alneberg @binnisb @inodb Are you still maintaining this project?

[alneberg]

@DavidGeeraerts, I'm listening at least.

I am not able to help out much at this point. I believe this is related to the bioconda recipe: https://github.com/bioconda/bioconda-recipes/tree/master/recipes/concoct which has been acting up several times over the years. I would be happy to merge in any fix if someone is able to find out what's going wrong.

[gwendolin]

Solved this issue by navigating to the bin and installing the mkl library: /home/science/miniconda3/envs/concoct_env/bin pip install mkl

Then everything works!

[mant-ux]

@gwendolin Hello！I have the same problem as you after install it by Bioconda. image I have follow you /home/science/miniconda3/envs/concoct_env/bin pip install mkl and run conda install concoct samtools ,But it still reported an error. How did you run it successfully? Can you give me some help? I'm confused with vbgmm, Thank you very much.

[gwendolin]

@mant-ux you will need to install the mkl library in the bin inside your conda environment. Your filepath won’t be the exact same as mine.