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Fast Linear Interacting Peptides Predictor
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Reference for b-factor normalization #1

Closed gezmi closed 2 years ago

gezmi commented 2 years ago

Hi,

I am looking at B-factor normalizations, I have seen such in FLIPPER but could not find any reference for the used method in the paper. I looked at Mobi2.0 which described the normalization however, without any reference. I could not yet find similar in the literature. Did I overlook something? What is the theory behind this kind of B-factor normalization?

Thank you for the help!

damianopiovesan commented 2 years ago

Hi,

The mapping between the resolution and Wilson_B was taken from here. They cite Popov et al. Acta Crystallogr. D. 2003.

Actually we used a very conservative strategy, we set 1.0 (max high temperature) to residues with a B-factor 4 times higher than average at that resolution. Those below the threshold are scaled linearly.

All the best

gezmi commented 2 years ago

Thank you!!!