Closed pmoulos closed 4 years ago
Hi @pmoulos
Thanks for submitting your package. We are taking a quick look at it and you will hear back from us soon.
The DESCRIPTION file for this package is:
Package: metaseqR2
Type: Package
Title: An R package for the analysis and result reporting
of RNA-Seq data by combining multiple statistical
algorithms
Author: Panagiotis Moulos <moulos@fleming.gr>
Maintainer: Panagiotis Moulos <moulos@fleming.gr>
Depends:
R (>= 4.0.0),
DESeq,
limma,
qvalue
Imports:
ABSSeq,
baySeq,
Biobase,
BiocParallel,
biomaRt,
Biostrings,
corrplot,
DESeq2,
DSS,
DT,
EDASeq,
edgeR,
GenomeInfoDb,
GenomicAlignments,
GenomicFeatures,
GenomicRanges,
gplots,
graphics,
grDevices,
heatmaply,
htmltools,
httr,
IRanges,
jsonlite,
log4r,
NOISeq,
magrittr,
methods,
NBPSeq,
pander,
parallel,
rmarkdown,
rmdformats,
Rsamtools,
RSQLite,
rtracklayer,
S4Vectors,
splines,
stats,
stringr,
SummarizedExperiment,
survcomp,
utils,
VennDiagram,
vsn,
zoo
Suggests:
BiocGenerics,
BiocManager,
BSgenome,
knitr,
RMySQL,
RUnit
Enhances:
TCC
Description: Provides an interface to several normalization and
statistical testing packages for RNA-Seq gene expression data.
Additionally, it creates several diagnostic plots, performs
meta-analysis by combinining the results of several statistical
tests and reports the results in an interactive way.
License: GPL (>= 3)
Encoding: UTF-8
LazyLoad: yes
LazyData: yes
URL: http://www.fleming.gr
biocViews: Software, GeneExpression, DifferentialExpression, WorkflowStep,
Preprocessing, QualityControl, Normalization, ReportWriting, RNASeq,
Transcription, Sequencing, Transcriptomics, Bayesian, Clustering,
CellBiology, BiomedicalInformatics, FunctionalGenomics, SystemsBiology,
ImmunoOncology, AlternativeSplicing, DifferentialSplicing,
MultipleComparison, TimeCourse, DataImport
VignetteBuilder: knitr
Authors@R: c(person(given="Panagiotis", family="Moulos",
email="moulos@fleming.gr", role=c("aut", "cre")))
Version: 0.0.21
Date: 2020-03-25
Collate:
'annotation.R'
'argcheck.R'
'count.R'
'metaseqr2-data.R'
'export.R'
'filter.R'
'json.R'
'main.R'
'meta.R'
'norm.R'
'metaseqR2-package.R'
'plot.R'
'query.R'
'reportdb.R'
'sim.R'
'stat.R'
'tracks.R'
'util.R'
'zzz.R'
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@pmoulos can you explain the relationship between this package and metaseqR ? Usually it is counter-productive to have two versions of a package available. Why not just increment your existing package to version 1.99.0, 1.99.1, ... until the release, when it will become version 2.0.0 ?
Dear Package contributor,
This is the automated single package builder at bioconductor.org.
Your package has been built on Linux, Mac, and Windows.
On one or more platforms, the build results were: "ERROR". This may mean there is a problem with the package that you need to fix. Or it may mean that there is a problem with the build system itself.
Please see the build report for more details.
Received a valid push; starting a build. Commits are:
4486b85 Fixed 1st submission check errors
@mtmorgan, there is a relationship between the two packages similarly with the case of DESeq and DESeq2. metaseqR2 has some similar logic to metaseqR but it has major and breaking additions, different calculation models and severe refactoring, including changes in argument naming, exported functions and many other aspects. Therefore, it would be easier for users to educate themselves on a new, simpler in terms of usage, but at the same time richer in functionalities package, than breaking many functionalities of the previous one.
In addition to the above, here is a summary of severe changes which to my opinion justify a new package:
Therefore, my logic is to maintain both packages:
I suggest that you deprecate metaseqR in the devel branch immediately after the next release. See for instance the problems with DESeq in the thread here: https://support.bioconductor.org/p/129152
Dear Package contributor,
This is the automated single package builder at bioconductor.org.
Your package has been built on Linux, Mac, and Windows.
On one or more platforms, the build results were: "WARNINGS". This may mean there is a problem with the package that you need to fix. Or it may mean that there is a problem with the build system itself.
Please see the build report for more details.
@mtmorgan, yes this can be done. In the meanwhile, would it be a problem for the two packages to co-exist in a release? Would it pose a problem for the current submission? Thanks.
we can continue with the current submission.
Received a valid push; starting a build. Commits are:
28425f3 Removed Author/Maintainer fields and left Authors@...
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Congratulations! The package built without errors or warnings on all platforms.
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Received a valid push; starting a build. Commits are:
cd787e2 Fixes one of the NOTEs in check
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Received a valid push; starting a build. Commits are:
9d7a965 Fixed typo causing DSS no replicates warning
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Received a valid push; starting a build. Commits are:
8d2eab1 Fixed issue with DE heatmaps in z-score mode and t...
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da6d247 Removed R version dependency and fully imported sp...
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a20bdd9 Bump version for rebuild
Dear Package contributor,
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Received a valid push; starting a build. Commits are:
dbe29cf Try to reduce check timings
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Hi @Kayla-Morrell thanks for looking at the package. Is there anything else required from my side given that the upcoming release package inclusion deadline is approaching?
@pmoulos - Thanks for checking in, I'm working on getting the initial review posted by the end of today. Hopefully that will be enough time for you to work on the changes and we can get it in by the deadline.
Hello @pmoulos,
Thank you for submitting to Bioconductor. Please see the initial review of the package below. The required changes must be made while the suggested changes do not have to be but we strongly encourage them. Comment back here with updates that have been made and when the package is ready for a re-review. Keep in mind that the deadline for accepting new packages into the Bioconductor 3.11 release is 4/22.
[ ] REQUIRED: 'LazyLoad' isn't a needed field and should be removed.
[ ] REQUIRED: 'LazyData' should be set to false. It's out experience that when this is set to true it can slow down the loading of packages, especially those with data.
[ ] SUGGESTION: Consider adding these automatically suggested biocViews:
[ ] SUGGESTION: It is encouraged to include the 'BugReports:' field with a relevant link to GitHub for reporting Issues.
Well organized.
metaseqr2-annotation
eval=FALSE
:if(!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("metaseqR2")
[ ] SUGGESTION: We strongly encourage the use of table of contents.
[ ] REQUIRED: The last section of the vignette should be 'Session Info' and
should include sessionInfo()
.
[ ] REQUIRED: I'm not able to get this vignette building locally. I hope that once you change the inline coding the builders will pick up the issues I'm experiencing. But until then I'll work more on my side to see if I can debug some more. (It seems to error out from line 180-238).
metaseqr2-statistics
eval = FALSE
:if(!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("metaseqR2")
[ ] SUGGESTION: We strongly encourage the use of table of contents.
[ ] REQUIRED: Line 350, data should not be downloaded from Google Drive. The data should be part of the package, be coming from one of our Hubs, or from reliable sources.
[ ] REQUIRED: There should not be eval=FALSE
sections in the vignette, this
defeats the purpose of having executable code.
[ ] REQUIRED: The last section of the vignette should be 'Session Info' and
should include sessionInfo()
.
[ ] REQUIRED: While trying to run the vignette code locally I ran into a few
warnings that weren't shown in the vignettes themselves because you set
warnings=FALSE
. Please investigate the use of these functions
GenomeInfoDb::fetchExtendedChromInfoFromUCSC is deprecated
and
Specifying width/height in layout() in now deprecated
.
[ ] REQUIRED: Unexported object imported by ':::' calls. You are not allowed to access non-exported functions from other packages. Either ask the maintainer to export the function or write your own version of it using exported functions.
[ ] REQUIRED: There are ':::' calls to the package's namespace in its code. A package almost never needs to use ':::' for its own objects:
[ ] REQUIRED: install, biocLite, install.packages, or update.packages found in R files. Generally you shouldn't be making a call to these functions in your code.
[ ] REQUIRED: Avoid sapply(); use vapply() if possible. Found in files:
[ ] REQUIRED: Avoid 1:...; use seq_len() or seq_along() instead, if possible. Found in files:
[ ] REQUIRED: We do not allow for set.seed()
to be used in code. This
includes using setting it as a global variable. Please remove this.
[ ] SUGGESTION: For formating reasons, consider shorter lines. There are 23 lines that are > 80 characters long.
[ ] SUGGESTION: For formating reasons, consider multiples of 4 spaces for line indents. There are 417 lines that are not.
Best, Kayla
Hello @Kayla-Morrell, thank you for the review. Below, points addressed, answers and some comments:
DESCRIPTION
- [X] REQUIRED: 'LazyLoad' isn't a needed field and should be removed.
- [X] REQUIRED: 'LazyData' should be set to false. It's out experience that when this is set to true it can slow down the loading of packages, especially those with data.
- [X] SUGGESTION: Consider adding these automatically suggested biocViews:
- ATACSeq, Microarray, Metabolomics, Proteomics, Epigenetics, Cheminformatics, Regression, ExonArray, ChIPSeq, OneChannel, TwoChannel, MicroRNAArray, mRNAMicroarray, ProprietaryPlatforms, GeneSetEnrichment, BatchEffect
- [X] SUGGESTION: It is encouraged to include the 'BugReports:' field with a relevant link to GitHub for reporting Issues.
CITATION
- [X] REQUIRED: The 'mheader' for the CITATION file explains how to cite metaseqR. This should be changed to metaseqR2.
Vignettes
- [X] REQUIRED: Inline code should have single backticks
, yours currently have three which indicates blocks of code to be run. Please change all inline code to single backticks.
metaseqr2-annotation
- [X] REQUIRED: There should be an installation section that demonstrates to the user how to install the package from Bioconductor. The code should look like the following and should include
eval=FALSE
:if(!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("metaseqR2")
- [ ] SUGGESTION: We strongly encourage the use of table of contents.
- [X] REQUIRED: The last section of the vignette should be 'Session Info' and should include
sessionInfo()
.- [ ] REQUIRED: I'm not able to get this vignette building locally. I hope that once you change the inline coding the builders will pick up the issues I'm experiencing. But until then I'll work more on my side to see if I can debug some more. (It seems to error out from line 180-238).
metaseqr2-statistics
- [X] REQUIRED: There should be an installation section that demonstrates to the user how to install the package from Bioconductor. The code should look like the following and should include
eval = FALSE
:if(!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("metaseqR2")
- [ ] SUGGESTION: We strongly encourage the use of table of contents.
- [X] REQUIRED: Line 350, data should not be downloaded from Google Drive. The data should be part of the package, be coming from one of our Hubs, or from reliable sources.
- [X] REQUIRED: There should not be
eval=FALSE
sections in the vignette, this defeats the purpose of having executable code.- [X] REQUIRED: The last section of the vignette should be 'Session Info' and should include
sessionInfo()
.
BiocStyle
. Do you mean a TOC linking to all vignettes? How can I do that?Man pages
- [X] REQUIRED: There needs to be runnable examples in the following man pages which document exported object. If the example is not feasible the function needs to be tested somehow, where that be in a code chunk in a vignette or by using tests.
- createSignalTracks.Rd
- metaTest.Rd
- read2count.Rd
- readTargets.Rd
- statBayseq.Rd
inst/extdata
. These files were the basis for creating runnable examples for createSignalTracks.Rd, read2count.Rd, readTargets.Rd.R code
- [X] REQUIRED: While trying to run the vignette code locally I ran into a few warnings that weren't shown in the vignettes themselves because you set
warnings=FALSE
. Please investigate the use of these functionsGenomeInfoDb::fetchExtendedChromInfoFromUCSC is deprecated
andSpecifying width/height in layout() in now deprecated
.- [X] REQUIRED: Unexported object imported by ':::' calls. You are not allowed to access non-exported functions from other packages. Either ask the maintainer to export the function or write your own version of it using exported functions.
- 'GenomicFeatures:::GFF_FEATURE_TYPES'
- 'biomaRt:::generateFilterXML'
- 'biomaRt:::.setResultColNames'
- 'biomaRt:::martCheck'
- 'biomaRt:::martDataset'
- 'biomaRt:::martHost'
- 'biomaRt:::martVSchema'
- [X] REQUIRED: There are ':::' calls to the package's namespace in its code. A package almost never needs to use ':::' for its own objects:
- '.chromInfoFromBAM'
- '.chromInfroToSeqInfoDf'
- 'cmclapply'
- 'getUcscOrganism'
- 'getValidChrs'
- [ ] REQUIRED: install, biocLite, install.packages, or update.packages found in R files. Generally you shouldn't be making a call to these functions in your code.
- annotation.R, line 1907
- [X] REQUIRED: Avoid sapply(); use vapply() if possible. Found in files:
- annotation.R, lines 1240, 1298, and 1357
- json.R, line 2189
- main.R, lines 946, 950, 1075, and 1079
- meta.R, lines 350 and 358
- plot.R, lines 2057 and 2766
- sim.R, lines 361, 388, 389, 390, 416, 416, and 418
- stat.R, lines 14, 307, and 653
- tracks.R, lines 99 and 222
- util.R, lines 2968 and 2969
- [X] REQUIRED: Avoid 1:...; use seq_len() or seq_along() instead, if possible. Found in files:
- annotation.R, lines 154, 215, 268, 341, 716, 738, 1162, 1222, 1279, 1339, 1538, 1587, 1595, 1612, 1616, 1656, 1922, 2773, 2805, 2837, 2852, 2902, 2953, 2968, 2998, 3030, 3045, 3102, 3154, 3172, and 3203
- argcheck.R, line 6
- count.R, lines 218, 515, 551, and 583
- export.R, line 25
- json.R, lines 149, 232, 407, 441, 629, 835, 867, 868, 989, 1097, 1117, 1123, 1245, 1489, 1502, 1526, 2036, 2152, 2178, 2629, 2888, 3242, 3245, and 3257
- main.R, lines 788, 879, 1011, 1124, 1753, 1756, and 2095
- meta.R, lines 94, 126, 160, 271, 312, 341, 348, and 356
- norm.R, lines 12, 139, 166, 200, and 277
- plot.R, lines 9, 47, 57, 192, 266, 285, 365, 366, 461, 483, 484, 522 593, 594, 647, 649, 673, 675, 855, 904, 1257, 1275, 1317, 1318, 1457, 1582, 1805, 1810, 1860, 1865, 1875, 2093, 2158, 2206, 2231, 2639, 2658, 2677, 2791, 2806, 2814, 2815, 2828, 2829, 2837, 2838, 2851, 2852, 2952, 3116, 3118, 3126, 3143, 3159, and 3160
- reportdb.R, lines 422, 521, 538, 548, 563, 573, 601, 610, 720, 721, 731, 732, 810, 839, 864, 873, 912, 1001, and 1017
- sim.R, lines 12, 19, 28, 29, 56, 57, 102, 103, 125, 162, 164, 168, 170, 183, 188, 194, 201, 207, 210, 212, 238, 249, 250, 295, 323, 329, 346, 349, 354, 357, 359, 362, 378, and 406
- stat.R, lines 5, 113, 265, 437, 515, 524, 556, 566, 579, 592, 603, 613, 623, 642, 699, 781, 968, and 1284
- tracks.R, lines 245, 262, and 274
- util.R, lines 1588, 1613, 1621, 1638, 1647, 1670, 1738, 1832, 2805, 2810, 2819, 2829, 2839, and 2969
- [X] REQUIRED: We do not allow for
set.seed()
to be used in code. This includes using setting it as a global variable. Please remove this.- [X] SUGGESTION: For formating reasons, consider shorter lines. There are 23 lines that are > 80 characters long.
- [ ] SUGGESTION: For formating reasons, consider multiples of 4 spaces for line indents. There are 417 lines that are not.
GenomeInfoDb::fetchExtendedChromInfoFromUCSC is deprecated
, this is dependent on the version of Bioconductor and GenomeInfoDb
library on a system. Given that I would prefer the package to also run on older versions of R, I wrote a non-exported wrapper functions that checks the GenomeInfoDb
version and calls the most current function or the deprecated one in case of older versions of GenomeInfoDb
. This wrapper is expected to go away in future releases, e.g. when GenomeInfoDb::fetchExtendedChromInfoFromUCSC
becomes defunct.Specifying width/height in layout() in now deprecated
warning, this comes from the heatmaply
package and its relation to plotly (see also here. At this point I cannot do much about this other than removing the offending lines (e.g. 1902 at inst/metaseqr2_report.Rmd
) but then I can't control the size of my heatmaps which is not desired both for functional and aesthetic reasons.BiocManager::install()
(annotation.R, line 1907), I would like to keep this as an automation offered to the user. If a BSgenome package is missing, then instead of crashing/stopping, the function is trying to download and install it. I understand the good practices though. Would this be a particular problem in keeping it?set.seed()
, I need this for some plots in the report generated by the package. I removed it from zzz.R
and placed an advice in the main functionality vignette.Received a valid push; starting a build. Commits are:
0462f78 Version bump?
Dear Package contributor,
This is the automated single package builder at bioconductor.org.
Your package has been built on Linux, Mac, and Windows.
On one or more platforms, the build results were: "WARNINGS". This may mean there is a problem with the package that you need to fix. Or it may mean that there is a problem with the build system itself.
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Received a valid push; starting a build. Commits are:
91de3d6 Try to reduce check times
Dear Package contributor,
This is the automated single package builder at bioconductor.org.
Your package has been built on Linux, Mac, and Windows.
Congratulations! The package built without errors or warnings on all platforms.
Please see the build report for more details.
Hi @Kayla-Morrell, any hope to look at this :-)
@pmoulos - Thank you for making the necessary changes. I have just a couple follow up comments and then I should be ready to accept the package. Let me know when you are ready for me to look over these points.
Comments on DESCRIPTION
- Not all biocViews were added as the package clearly does not address them and may be misleading, specifically, Microarray, Metabolomics, Proteomics, Cheminformatics, ExonArray, OneChannel, TwoChannel, MicroRNAArray, mRNAMicroarray
That's fine that you've left some out from the list. These are automatically generated from BiocCheck based on the other biocViews that are listed.
Comments on CITATION
- An article describing metaseqR2 will be submitted this week. Should I change the reference now or wait until it gets published and then update the citation in dev?
As shown in our package guidelines, the citation is more so for how the package should be cited. I'll let you be the judge of if you want to leave it as is or not.
Comments on Vignettes
- Regarding the TOC, for each vignette, a TOC is generated by the
BiocStyle
. Do you mean a TOC linking to all vignettes? How can I do that?- I removed the example requiring to download data from Google Drive and replaced with additional external resources (GitHub wiki) for additional help on using the package.
- Regarding the vignette that you cannot build locally, the code in that particular chunk is platform-dependent. It will not run on Windows machines (but the code checks for that). Other than that, I can build it locally and it seems that the Bioconductor build system does not face an issue.
By adding the 'toc' in the top of the vignette code then TOC's will be put at the top of the individual vignettes (see the code below).
BiocStyle::html_document: toc: true toc_float: true
Comments on Man pages
- A small subset of 2 real BAM files was added in
inst/extdata
. These files were the basis for creating runnable examples for createSignalTracks.Rd, read2count.Rd, readTargets.Rd.- Runnable examples were added in metaTest.Rd and statBayseq.Rd
Thank you for adding the BAM files, there needs to be some documentation about these files. There needs to be documentation for the 'inst/extdata/' files in an 'inst/script/'. Please see our package guidelines here for what to include for the documentation.
Best, Kayla
Hi @Kayla-Morrell,
I added a README.txt
file in inst/script
with a description on the source of these files and addressed the TOC issue.
Hope this is OK.
Thank you for all the help.
Received a valid push; starting a build. Commits are:
90f9967 Address 2nd review
@pmoulos - Those look fine. Just one last last change that I can see. In the vignettes, line 11 (should be the same in the both files) should be:
%\VignetteEngine{knitr::rmarkdown}
this will help render the vignettes properly.
Dear Package contributor,
This is the automated single package builder at bioconductor.org.
Your package has been built on Linux, Mac, and Windows.
On one or more platforms, the build results were: "skipped, ERROR". This may mean there is a problem with the package that you need to fix. Or it may mean that there is a problem with the build system itself.
Please see the build report for more details.
Received a valid push; starting a build. Commits are:
3259ee1 Version bump
Hi @Kayla-Morrell, I changed the line in the vignettes. There was an error in the last built in Linux, however I suspect that this is related to Ensembl and its reduced functionality over the past few weeks... Let's see how this built goes. Thanks.
Dear Package contributor,
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Your package has been built on Linux, Mac, and Windows.
Congratulations! The package built without errors or warnings on all platforms.
Please see the build report for more details.
Your package has been accepted. It will be added to the Bioconductor Git repository and nightly builds. Additional information will be posed to this issue in the next several days.
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The master branch of your GitHub repository has been added to Bioconductor's git repository.
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(Builds sometimes fail, so ensure that the date stamps on the main landing page are consistent with the addition of your package). Once the package builds successfully, you package will be available for download in the 'Devel' version of Bioconductor using BiocManager::install("metaseqR2")
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