Closed lhc70000 closed 6 months ago
Hi @lhc70000
Thanks for submitting your package. We are taking a quick look at it and you will hear back from us soon.
The DESCRIPTION file for this package is:
Package: scMultiSim
Title: Simulation of Multi-Modality Single Cell Data Guided By Gene Regulatory Networks and Cell-Cell Interactions
Version: 0.99.0
Authors@R:
c(person(given = "Hechen",
family = "i",
role = c("aut", "cre"),
email = "hli691@gatech.edu",
comment = c(ORCID = "0000-0003-4907-429X")),
person(given = "Xiuwei",
family = "Zhang",
email = "zhangxiuwei03@gmail.com",
role = "aut"),
person(given = "Michael",
family = "Squires",
email = "squiresmf@gatech.edu",
role = "aut"))
Description:
scMultiSim simulates paired single cell RNA-seq, single cell ATAC-seq and RNA velocity data,
while incorporating mechanisms of gene regulatory networks, chromatin accessibility and
cell-cell interactions. It allows users to tune various parameters controlling the
amount of each biological factor, variation of gene-expression levels,
the influence of chromatin accessibility on RNA sequence data, and so on.
It can be used to benchmark various computational methods for single cell multi-omics data,
and to assist in experimental design of wet-lab experiments.
License: Artistic-2.0
Encoding: UTF-8
RoxygenNote: 7.2.3
Depends:
R (>= 3.5.0)
Imports:
foreach,
rlang,
dplyr,
ggplot2,
Rtsne,
ape,
MASS,
matrixStats,
phytools,
SummarizedExperiment,
KernelKnn,
gplots,
zeallot,
crayon,
assertthat,
igraph,
methods,
grDevices,
graphics,
stats,
utils
Suggests:
knitr,
rmarkdown,
roxygen2,
testthat (>= 3.0.0)
biocViews: SingleCell, Transcriptomics
VignetteBuilder: knitr
Roxygen: list(markdown = TRUE)
BugReports: https://github.com/ZhangLabGT/scMultiSim/issues
Config/testthat/edition: 3
* checking re-building of vignette outputs ... ERROR
Error(s) in re-building vignettes:
...
--- re-building ‘basics.Rmd’ using knitr
Error: processing vignette 'basics.Rmd' failed with diagnostics:
there is no package called ‘markdown’
--- failed re-building ‘basics.Rmd’
--- re-building ‘spatialCCI.rmd’ using knitr
Quitting from lines 75-94 [unnamed-chunk-1] (spatialCCI.rmd)
Error: processing vignette 'spatialCCI.rmd' failed with diagnostics:
object 'GRN_params_100' not found
--- failed re-building ‘spatialCCI.rmd’
SUMMARY: processing the following files failed:
‘basics.Rmd’ ‘spatialCCI.rmd’
Error: Vignette re-building failed.
Execution halted
if you need markdown, please declare in DESCRIPTION. fix the data availability error. thanks
Thank you very much. I have updated the repo to fix these two problems.
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Hi, I'm looking forward to reviewing the package! Please fix the build errors before I can proceed.
Hi @ococrook! I'm so sorry for the delay. I wanted to resolve the timeout issue; however, since scMultiSim is a data simulator, some simulation code in the vignette may take a long time to run (~10 minutes). I'm considering either
Which one do you prefer, or is there any other solution you would recommend? Thanks!
Hi!
How big the demo data - this maybe your limitation if it over 5mb you would have to store it in a data package. I favour the first option if it makes sense to do that - and allow your user to locally increase the number of cells and genes easily.
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Hi @ococrook, sorry for the delay! Now I'll prioritize this package and all build errors have been fixed. I will also look into the warnings soon.
I'm committed to promptly addressing any review comments. Thank you for your time with this large package!
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Hi @ococrook! The package now builds without errors or warnings. I apologize once more for the delay caused by my personal issues. I look forward to your review and will promptly address any comments.
Thanks! Sorry I didnt see the notification. I'll get to this shortly
This is great! A few things to tidy up:
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Are you able to fix the timeout?
The problem this time was not the timeout…… My unit test failed on BioConductor's build server after I pushed the new changes.
Running the tests in tests/testthat.R failed.
Last 20 lines of output:
`actual[3:15]`: 18 33 88 0 2 0 24 72 6 7 and 3 more...
`expected[3:15]`: 18 33 88 5 4 0 8 96 0 18 ...
Failure ('test-1_main.R:151:3'): simulates spatial data with discrete population and HGE
res$counts[selectedIndicies] (`actual`) not equal to c(...) (`expected`).
actual | expected
[1] 116.691 - 109.069 [1]
[2] 59.994 - 60.415 [2]
[3] 85.398 - 91.912 [3]
[4] 1.082 - 0.582 [4]
[5] 172.034 - 177.674 [5]
[6] 202.649 - 197.066 [6]
[7] 95.052 - 102.315 [7]
[8] 56.943 - 65.998 [8]
[9] 96.683 - 89.161 [9]
[10] 2.693 - 3.173 [10]
... ... ... and 7 more ...
[ FAIL 7 | WARN 0 | SKIP 0 | PASS 5 ]
Error: Test failures
This is really strange since these tests successfully pass on my macOS and Linux machines. I'm still trying to figure it out; do you have any thoughts?
Odd, usually a package dependcy or perhaps random seed issue?
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Hi @ococrook, I have resolved the review comments and passed the Bioc Build System. Could you have a look? Thanks!
@lshep where are the old build reports contained? I think we can accept this one shortly after double check.
Sorry for the delay on my end - working only part time at the moment.
This is a very clear package as a I noted before.
Im keen to see some of the easy Notes cleared up e.g. vapply, 1:N constructions etc, corrected R dependency.
Make sure to run Biochcheck. A few notes are fine but 13 is too many.
I realise not every package can depend on bioc infrastructure but could you give me a breif reason why this package does not depend on any bioconductor packages and why its more appropraite for the package on Bioconductor rather than CRAN?
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