Bioprotocols / labware-databank

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Intrinsic fluorescence as property? #7

Open photocyte opened 1 year ago

photocyte commented 1 year ago

I expect the current set of properties for labware (specifically microplates), includes things like color, if it is opaque or translucent, etc.

But I came across an unexpected additional property. Intrinsic fluorescence. Check out the middle Nunc Brand (Thermo Fisher) plate in the below photo. It clearly fluoresces a violet / blue under UV light (and sunlight, I first noticed it in front of a window), whereas the other plates don't show that fluorescence. They are all polypropylene plates, nominally with the exact same "specifications"

image

jakebeal commented 1 year ago

That's surprising!

Are they clear bottom plates, which one could use in a plate reader, or not? If not, then it probably doesn't actually matter... If so, then it depends what the behavior of the bottom of the well is...

photocyte commented 1 year ago

They are clear bottom, but V-bottom. I guess you could do a bottom-read fluorescence, but would be a bit weird & probably quite sensitive to the exact distance of the fiber optic probe. Top-reading fluorescence could use these plates though.

Should be clear: These the middle plate is a different product from the other two, despite their similarity of labeling.

markdoerr commented 1 year ago

@photocyte, this is also new to me, thanks a lot for the hint. I am afraid that we experience quite some surprises, if we look in more depth into the materials. Since we do not know all surprising properties, we could add a (only human readable) property, e.g. material properties (Type String), where such findings could be documented. If we would start modelling the fluorescence, we would need to specify the excitation and emission wavelength range - and some quantification of the intensity. I think, this we keep as a backlog for version 2.0.

photocyte commented 1 year ago

I agree, made a milestone for 2.0 & assigned this issue to it.