bug in the assembly primer function

BjornFJohansson / pydna

Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning.

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bug in the assembly primer function #22

Closed BjornFJohansson closed 8 years ago

BjornFJohansson commented 8 years ago

There seems to be a bug under certain conditions in the assembly primers function in the primer_design module. Avoid it use it until this is resolved.

BjornFJohansson commented 8 years ago

seems it is the correct primers returned, but in some cases the primers returned have too low tm.

BjornFJohansson commented 8 years ago

Specifically, in certain cases the annealing part of primers was too short. This has been fixed in 1.0.2