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BjornFJohansson / pydna

Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning.
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PCR not specific error report for a pair of specific primers #256

Closed JeffXiePL closed 2 months ago

JeffXiePL commented 2 months ago

244

Hello, I had been writing documentation for a Gibson Assembly example, and I couldn't figure out why I am getting this "PCR not specific!" error message. I have checked that my primer sequences are specific to the vector. I have linked my code snippet here, which includes the error message: https://github.com/JeffXiePL/pydna/blob/dca58765c5920f7da0641e0d7ce06e233b23bee8/docs/notebooks/Example_Gibson.ipynb#L538C3-L565C5.

Thank you, Peilun

manulera commented 2 months ago

Hi @JeffXiePL this was hard to debug for a newcomer! I document this in the PR https://github.com/JeffXiePL/pydna/pull/4

manulera commented 2 months ago

Closing as it's fixed already