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BjornFJohansson / pydna

Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning.
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Enable adding spacer to assembly_fragments #269

Closed manulera closed 1 month ago

manulera commented 1 month ago

Hi @BjornFJohansson,

I think it would be nice if we allowed adding spacers to the primers generated by assembly_fragments, so that they contain an extra sequence between the part that binds to the PCR template and the homology region. This could be an extra argument to assembly_fragments that by default is None and otherwise contains a list of the spacers that should go between sequences.

manulera commented 1 month ago

@hiyama341 maybe you have some thoughts on this?

hiyama341 commented 1 month ago

Hi Manu, this is such a good idea that will really make some of the things that we are doing easier. For many projects, I have had to append these spacers so this is super helpful. Have you set a limit to the length of spacer you would incorporate into the primers btw? Looking forward to hearing from you.

BjornFJohansson commented 1 month ago

This works, see #288