BodenmillerGroup / steinbock

A toolkit for processing multiplexed tissue images
https://bodenmillergroup.github.io/steinbock
MIT License
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Error: No panel/.mcd/.txt file found #242

Closed icepsi23 closed 5 months ago

icepsi23 commented 5 months ago

I'm using this example to run steinbock: https://bodenmillergroup.github.io/IMCDataAnalysis/prerequisites.html

I'm at:

raw data preprocessing

steinbock preprocess imc panel --namecol Clean_Target

After running that line I got the: Error: No panel/.mcd/.txt file found

Any advise on how I can fix this problem? I'm new at using a docker and new to analyzing IMC data.

Screenshot 2024-02-13 at 10 39 45 AM
Milad4849 commented 5 months ago

Hi @icepsi23

It appears that you are missing some files or that they are not in the expected location. Please check that you have the necessary files at expected locations, as specified here. If you execute all commands in 4.3 successfully, that should be the case, therefore make sure all commands in 4.3, up the error causing line, are executed successfully.

icepsi23 commented 5 months ago

I actually have the panel file on the folder, and have run successfully run the 4.3 section successfully up until that line.

Screenshot 2024-02-14 at 8 05 16 AM
Milad4849 commented 5 months ago

In that case I am afraid your error is not reproducible, I ran 4.3 from scratch and everything works fine. Is the above the content of your raw folder? You can try to provide the path(s) to the relevant files explicitly: image

icepsi23 commented 5 months ago

Yes, the above is my raw folder. I figure out the problem was the linux support software that I was using. Thank you for your help.