Open Michael-Geuenich opened 3 years ago
Hi Michael,
Sorry for the late reply! Can you show me the settings in the run log, especially what is the est. fragment size in your run?
I suspect the est. fragment size is causing the issue. In the meanwhile, try setting -f 0
to treat it as DNase or ATAC-seq and see if any problem there for us to debug.
Sam
Hi,
I'm trying to run F-Seq2 on 3' RNA single end sequencing data to identify polyadenylation peaks. I'm running the following
However, this results in the following error:
Because the error mentioned distance I tried adding
-f 150
to my call. This made the program run longer (and hence I think further). However, then I got the following error:Ultimately I want to estimate the fragment size from the data though, rather than setting it. Is this possible?