Closed davebridges closed 9 years ago
FOS is downregulated, fold change is 0.196338468, q-age=0.011664068. No stats for FOSB. JUN is downregulated fold change is 0.379619591, q-age=0.013204632 JUNB is downregulated fold change is 0.334653136, q-age=0.037657139 JUND is unchanged
Together, Jun and Fos form AP1. Targets of AP1 are downregulated quite a bit based on GSEA.
AP1 is regulated by ERK1 (MAPK3) and ERK3 (MAPK1). MAPK3 is slightly reduced in expression. 15% reduced, p=0.081. MAPK1 is unchanged.
They saw upregulation, we see downregulation
Gurland G, Ashcom G, Cochran BH, Schwartz J. Rapid events in growth hormone action. Induction of c-fos and c-jun transcription in 3T3–F442A preadipocytes. Endocrinology. 1990;127:3187–3195.
Check TF activity from GSEA and levels by barplot and think about putting into the results/discussion