BrooksLabUCSC / flair

Full-Length Alternative Isoform analysis of RNA
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What SALMON method does FLAIR quantify use when called with --salmon? #183

Closed bayraktar1 closed 2 years ago

bayraktar1 commented 2 years ago

Hi,

When running FLAIR quantify you can specify --salmon to have the quantification done by SALMON.

The SALMON documentation mentions that there is a mapping-based and alignment-based method. What SALMON quantification method does FLAIR call when the --salmon flag is used?

I was not able to find an answer on the wiki.

Thanks,

bayraktar1 commented 2 years ago

From the published FLAIR paper: """We evaluated salmon as a quantification tool using its alignment-based mode with all minimap2 alignments, as performance of the quasi-alignment mode could suffer from low read accuracy."""