CAMI-challenge / CAMISIM

CAMISIM: Simulating metagenomes and microbial communities
https://data.cami-challenge.org/participate
Apache License 2.0
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Error running simulation using custom genomes #169

Closed CassandraHjo closed 11 months ago

CassandraHjo commented 11 months ago

I am attempting to execute CAMISIM using custom genomes, but I get this error message:

image

This is my config file: image

This is my genome_to_id.tsv: image

This is my metadata.tsv: image

My primary interest lies in the sequenced reads generated by CAMISIM, rather than the gold standard or assembly outputs.

I attempted to replace the genome specified in line 3 of the genome_to_id file with an alternate genome, but this gives the same error as above. My input genomes are MAGs in .fasta format. Although the MAGs do not represent complete genomes, the sequences within the .fasta files are contiguous. (I have checked that the simulation runs with the default files).

CassandraHjo commented 11 months ago

The issue with the 'genome_to_id.tsv' file originated from an incorrect formatting where tabs were inadvertently replaced with spaces. Upon rectifying this by reinstating tabs as the delimiter the simulation executed flawlessly without any errors.