CCBR / CARLISLE

Cut And Run Analysis Pipeline
https://ccbr.github.io/CARLISLE/
MIT License
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deseq2 sample check is failingi #126

Closed slsevilla closed 7 months ago

slsevilla commented 8 months ago

Describe the bug From Erica

hi Sam, good morning! I'm still running into some DESeq2 issues with CARLISLE that I wanted to run by you. All of my conditions have at least 3-4 replicates, but (Sh2 vs PC) are failing during the check for single-sample groups, while the other half (Sh1 vs PC) are being treated as having a single sample and producing empty output files. I think this might be because they have a "1" in the group name. I'm going to comment out the check for my projects, but just wanted to let you know!

Will need to design a better way to determine samples vs groups

epehrsson commented 8 months ago

_diff_markdown_wrapper.R, line 90 - log2FC is being assigned FDR cutoff