Closed kelly-sovacool closed 1 year ago
on an interactive node, running champagne with the biowulf profile (uses slurm executor for each process)
nextflow run main.nf -profile test_mm10,biowulf -resume
ERROR ~ Error executing process > 'CALL_PEAKS:GEM (CTCF_ChIP_MEF_p20_1)' Caused by: Process `CALL_PEAKS:GEM (CTCF_ChIP_MEF_p20_1)` terminated with an error exit status (255) Command executed: java -Xmx30g -jar $GEMJAR \ --t 12 \ --d Read_Distribution_default.txt \ --g mm10.fa.sizes \ --genome chr17.fa ref.fa chr9.fa chr13.fa chr19.fa chr8.fa chr12.fa chr7.fa chr14.fa chr6.fa chr5.fa chr_rDNA.fa chr15.fa splitRef.py chr4.fa chr3.fa chrY.fa chr10.fa chr16.fa chr2.fa chrX.fa chr18.fa chr1.fa chrom.sizes chr11.fa chrM.fa \ --expt CTCF_ChIP_MEF_p20_1.TagAlign.bed \ --ctrl WCE_p20.TagAlign.bed \ --out CTCF_ChIP_MEF_p20_1 \ --k_min 6 \ --k_max 13 \ --outNP \ --nrf Command exit status: 255 Command output: ============================ Round 0 ============================ Running with 12 threads ... 100 /2353272 23.7s 1000 /2353272 2.1m 10000 /2353272 2.6m 1000000 /2353272 6.7m 2000000 /2353272 6.7m 2353272 /2353272 6.7m Scaling condition , IP/Control = 0.84 --------------------------- Events discovered Significant: 61550 Insignificant: 537 Filtered: 3 Total 10723 homotypic events (within 501bp of other events). Finish binding event prediction: 6.7m Refine read distribution from 2000 binding events. ============================ Round 1 ============================ Running with 12 threads ... 100 /2353272 2.3s 1000 /2353272 3.3s 10000 /2353272 4.3s 1000000 /2353272 1.7m 2000000 /2353272 3.3m 2353272 /2353272 3.9m --------------------------- Events discovered Significant: 63921 Insignificant: 1135 Filtered: 0 Total 4650 homotypic events (within 545bp of other events). Finish binding event prediction: 4.0m Refine read distribution from 2000 binding events. Loading genome sequences ... Command error: WARNING: While bind mounting '/gpfs:/gpfs': destination is already in the mount point list chr11.fa genome sequence file is not found at directory chr17.fa. Work dir: /gpfs/gsfs12/users/sovacoolkl/champagne_mm10/work/57/bfcb8c6762e41a5131e578f84294fe
The problem is GEM takes a folder of chromosomes but we're currently giving it a space-delimited list of files.
on an interactive node, running champagne with the biowulf profile (uses slurm executor for each process)