CCBR / RENEE

A comprehensive quality-control and quantification RNA-seq pipeline
https://CCBR.github.io/RENEE/
MIT License
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Add QC check if no reads are left after `cutadapt` #118

Open slsevilla opened 9 months ago

slsevilla commented 9 months ago

Problem

Recently had a project that had no reads left after trimming. The sample did not fail and continued downstream making it difficult to pinpoint where the sample had truly failed

Solution

After cutadapt add a check to ensure there are suitable number of reads to move on in the pipeline.

kelly-sovacool commented 6 months ago

this can happen if a file contains only adapters -- make a unit test with adapters

kelly-sovacool commented 1 week ago

add code to cutadapt rule. if the file is empty, throw an error