CCBR / RENEE

A comprehensive quality-control and quantification RNA-seq pipeline
https://CCBR.github.io/RENEE/
MIT License
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feat: add hg38 v45 reference genome #127

Closed kelly-sovacool closed 6 months ago

kelly-sovacool commented 6 months ago

Changes

Adds configuration files for gencode release 45 of hg38.

Steps

Using PRI regions

build command

renee build \
    --sif-cache /data/CCBR_Pipeliner/SIFS \
    --ref-fa GRCh38.primary_assembly.genome.fa \
    --ref-name hg38 \
    --ref-gtf gencode.v45.primary_assembly.annotation.gtf \
    --gtf-ver 45 \
    --output /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45

change ownership

chown -R :CCBR_Pipeliner /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45
chmod -R a+rX /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45

testing

biowulf ✅

/data/CCBR_Pipeliner/Pipelines/RENEE/renee-dev-sovacool/bin/renee run \
    --input /data/CCBR_Pipeliner/Pipelines/RENEE/develop/.tests/*.R1.fastq.gz \
    --genome hg38_45 \
    --mode slurm \
    --output /data/$USER/renee_test_gencode-45 \
    --sif-cache /data/CCBR_Pipeliner/SIFS

frce [in progress]

/mnt/projects/CCBR-Pipelines/pipelines/RENEE/renee-dev-sovacool/bin/renee run \
    --input latest/.tests/*.R?.fastq.gz \
    --output /scratch/cluster_scratch/$USER/renee_test_gencode-45 \
    --genome hg38_45 \
    --mode slurm \
    --tmp-dir /scratch/cluster_scratch/$USER \
    --sif-cache /mnt/projects/CCBR-Pipelines/SIFs

Issues

Resolves #126

PR Checklist

(~Strikethrough~ any points that are not applicable.)

kopardev commented 6 months ago

Is fasta different from the one in /gpfs/gsfs10/users/CCBR_Pipeliner/Pipelines/RENEE/resources/fastas ?

kelly-sovacool commented 6 months ago

Is fasta different from the one in /gpfs/gsfs10/users/CCBR_Pipeliner/Pipelines/RENEE/resources/fastas ?

I had assumed they would be different, but they are actually the same. I'll switch it to use the existing one.

kelly-sovacool commented 6 months ago

this is ready @kopardev