CCRGeneticsBranch / khanlab_ngs_pipeline

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snpEff -db error #18

Closed slsevilla closed 1 year ago

slsevilla commented 1 year ago

Sample

SJ000026/SJRHB026_R ngs_pipeline_SJ000026=SJRHB026_R=20220911

Error:

/data/khanlab2/processed_DATA/ngs_pipeline_SJ000026=SJRHB026_R=20220911_20221220_005856.log
Error in rule SNPEff:
Error executing rule SNPEff on cluster (jobid: 29, external: 54653552, jobscript: /gpfs/gsfs10/users/khanlab2/processed_DATA/.snakemake/tmp.3mgq7an1/SNPEff.29). For error details see the cluster log and the log files of the involved rule(s).
Error in rule SNPEff:
Error executing rule SNPEff on cluster (jobid: 29, external: 54653860, jobscript: /gpfs/gsfs10/users/khanlab2/processed_DATA/.snakemake/tmp.3mgq7an1/SNPEff.29). For error details see the cluster log and the log files of the involved rule(s).
ssh -o StrictHostKeyChecking=no biowulf.nih.gov "echo 'ngs-pipeline version v4.2 failed on SJ000026. Error occured on biowulf.nih.gov. Working Dir:  /data/khanlab2/processed_DATA/' |mutt -s 'Khanlab ngs-pipeline Status' `whoami`@mail.nih.gov  -c gangalapudiv2@nih.gov -c sevillas2@nih.gov -c wenxi@mail.nih.gov "

Shell command is missing important component dbnsfp:

java -Xmx${{MEM}}g -Djava.io.tmpdir=${{LOCAL}} -jar $SNPEFF_JARPATH/SnpSift.jar \
        dbnsfp -db {input.db} \
        -c {params.snpEff_config} \
        -a {input.vcf} | \
        java -Xmx${{MEM}}g -jar $SNPEFF_JARPATH/snpEff.jar \
        -t \
        -canon {params.snpEff_genome} > {output.eff}

Code has been updated on Github and on the local instance of the pipeline /data/Clinomics/Tools/ngs_pipeline_4.2_dev/

slsevilla commented 1 year ago

completed with commit a145acd5fd7f08a9baf31c4784d76a5e0fef73fb