Open DOH-JDJ0303 opened 1 year ago
Thanks for your comment. Is it giving issues when you run on the cluster > At our end, snpEff is running properly on the cluster. Unfortunately, we don't have access to the cloud to test it.
Hi @urbagal, this issue is only experienced when running MycoSNP on the cloud and might be specific to Nextflow Tower and/or AWS Batch. As described above, the problem is that the SnpEff config files are passed via the $args variable, rather than being passed as input to the process. This means that Nextflow does not stage the files, which is necessary when using Nextflow Tower or AWS batch. I submitted a pull request that addresses this issue #103.
Oh, sorry I missed it. Thanks.
Describe the bug SNPEFF_ANN fails due to SnpEff being unable to read the config file (snpEff.config). I believe this is because it is being passed in the $args, rather than as an input, so the file name is being passed verbatim and is not actually being localized within the work directory (see log below). This was run using MycoSNP v1.5 (from GitHub, not local installation) on Nextflow Tower using an AWS Batch compute environment and an AWS S3 bucket. The command from nf-tower is below:
Impact Unable to run MycoSNP with SnpEff option, which means no detection/reporting of variants.
To Reproduce Steps to reproduce the behavior:
Logs Note: AWS bucket paths have been modified, but the general format remains the same. ''' The exit status of the task that caused the workflow execution to fail was: 255
Error executing process > 'NFCORE_MYCOSNP:MYCOSNP:SNPEFF:SNPEFF_ANN (combined)'
Caused by: Essential container in task exited
Command executed:
snpEff \ -Xmx16g \ -config s3://bucket-name/nextflow/mycosnp/snpEff.config \ -v candida_auris_gca_016772135.1 \ -csvStats combined.csv \ finalfiltered.vcf.gz \
cat <<-END_VERSIONS > versions.yml "NFCORE_MYCOSNP:MYCOSNP:SNPEFF:SNPEFF_ANN": snpeff: $(echo $(snpEff -version 2>&1) | cut -f 2 -d ' ') END_VERSIONS
Command exit status: 255
Command output: (empty)
Command error: Picked up _JAVA_OPTIONS: -Djava.io.tmpdir=./ 00:00:00 SnpEff version SnpEff 4.3t (build 2017-11-24 10:18), by Pablo Cingolani 00:00:00 Command: 'ann' 00:00:00 Reading configuration file 's3://bucket-name/nextflow/mycosnp/snpEff.config'. Genome: 'candida_auris_gca_016772135.1' 00:00:00 Reading config file: /tmp/nxf.XXXXhKEuMs/s3:/bucket-name/nextflow/mycosnp/snpEff.config 00:00:00 Reading config file: /usr/local/share/snpeff-4.3.1t-5/s3:/bucket-name/nextflow/mycosnp/snpEff.config java.lang.RuntimeException: Cannot read config file 's3://bucket-name/nextflow/mycosnp/snpEff.config' at org.snpeff.snpEffect.Config.readProperties(Config.java:704) at org.snpeff.snpEffect.Config.readProperties(Config.java:716) at org.snpeff.snpEffect.Config.readConfig(Config.java:592) at org.snpeff.snpEffect.Config.init(Config.java:480) at org.snpeff.snpEffect.Config.(Config.java:117)
at org.snpeff.SnpEff.loadConfig(SnpEff.java:451)
at org.snpeff.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1000)
at org.snpeff.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:984)
at org.snpeff.SnpEff.run(SnpEff.java:1183)
at org.snpeff.SnpEff.main(SnpEff.java:162)
00:00:00 Logging
00:00:01 Done.
Work dir: s3://bucket-name/nextflow/e6/e4bbc87323a4033e379f740e24c897
Tip: when you have fixed the problem you can continue the execution adding the option
-resume
to the run command line '''