CERI-KRISP / SARS-CoV-2-epidemic-in-Africa

Expanding Africa SARS-CoV-2 sequencing capacity in a fast evolving pandemic.
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where to find the "metadata_2022-03-30_23-13.tsv" file? #2

Closed liamxg closed 2 months ago

jsan4christ commented 1 year ago

Hi Liam,

This is GISAID metadata, the policy does not allow sharing it directly, one has to get the ids and download from GISAID after accepting the user policy. Also this file is particularly large, i.e. great than 100M so cannot be uploaded to a standard GitHub repo.

koppk commented 1 year ago

Hi Liam, Nextstrain_clade is a column header if you download metadata "as for input in Augur pipeline" from GISAID (not "as sequencing technology metadata"). Often you will find a "?" but you could replace that with values you might generate yourself by running the corresponding fasta sequences through Nextclade (standalone or webserver-based: https://clades.nextstrain.org/). Cheers, Katharina

jsan4christ commented 1 year ago

Yes that's correct. Sorry for some reason I thought the two of you were the same person.

On Sun, Oct 9, 2022, 4:37 AM Liam @.***> wrote:

@koppk https://github.com/koppk thanks for your help. many analysis is not familiar to me, hope you can help me on this.

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jsan4christ commented 1 year ago

That would be against the GISAID T&C but I will create an EPI_SET ID and share here.

On Sun, Oct 9, 2022 at 11:27 AM Liam @.***> wrote:

@jsan4christ https://github.com/jsan4christ can you share the meta data file for me, thanks.

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jsan4christ commented 1 year ago

Correct, but here is the Epi set;

EPI_SET ID: | EPI_SET_221009wd doi: | 10.55876/gis8.221009wd


EPI_SET ID: EPI_SET_221009wd doi: 10.55876/gis8.221009wd

jsan4christ commented 1 year ago

Hi Liam,

I believe that is a field you derive. You can generate by running the sequences through Nextclade and merging that field. The Nextstrain team keep changing the format of their output by dropping or renaming some fields and adding others. Sometimes we also add or derive depending on the needs of the analysis.

On Thu, Sep 22, 2022 at 4:57 AM Liam @.***> wrote:

@jsan4christ https://github.com/jsan4christ can not find Nextstrain_clade in Fig1.R.

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The Lord is my shepherd, I shall not want! Psalms 23

jsan4christ commented 1 year ago

No problem,

Any time.

On Tue, Oct 11, 2022 at 10:15 AM Liam @.***> wrote:

@jsan4christ https://github.com/jsan4christ thanks for your help.

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The Lord is my shepherd, I shall not want! Psalms 23

jsan4christ commented 1 year ago

@.***

On Tue, Oct 11, 2022 at 10:29 AM Liam @.***> wrote:

@jsan4christ https://github.com/jsan4christ can I get your email, for cooperation?

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The Lord is my shepherd, I shall not want! Psalms 23