I was wondering which type of fasta file should be provided to minnow estimate.
So far I have built a salmon index and ran alevin using what I would call a 'combined' fasta that has both transcript sequences and decoy sequences included.
I am wondering if minnow estimate needs the combined fasta, like alevin would use, or the fasta with just the transcript sequences (similar to what minnow index expects).
Hello,
I was wondering which type of fasta file should be provided to
minnow estimate.So far I have built a salmon index and ran alevin using what I would call a 'combined' fasta that has both transcript sequences and decoy sequences included.
I am wondering if
minnow estimateneeds the combined fasta, like alevin would use, or the fasta with just the transcript sequences (similar to whatminnow indexexpects).