I have a issue with the simulation of single cell dataset using 3'tag.
I observe that whatever parameter I used in the minnow simulate command (even the option --polyA), I simulate reads around a fixed distance from transcripts end of 400 bp.
How should I process to simulate reads to a closer distance from the end ? (10bp, 50bp or 100/150bp)
This will be very useful in order to get a simulated dataset with reads position reflecting the reads position of my inital experimental 3'tag single cell dataset.
Dear All,
Thanks first for this very useful tool.
I have a issue with the simulation of single cell dataset using 3'tag. I observe that whatever parameter I used in the minnow simulate command (even the option --polyA), I simulate reads around a fixed distance from transcripts end of 400 bp. How should I process to simulate reads to a closer distance from the end ? (10bp, 50bp or 100/150bp) This will be very useful in order to get a simulated dataset with reads position reflecting the reads position of my inital experimental 3'tag single cell dataset.
Thanks in advance Franz