COMBINE-lab / salmon

🐟 🍣 🍱 Highly-accurate & wicked fast transcript-level quantification from RNA-seq reads using selective alignment
https://combine-lab.github.io/salmon
GNU General Public License v3.0
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Error running salmon #914

Open bali-eng opened 9 months ago

bali-eng commented 9 months ago

I am running salmon and I am getting this error.

salmon (mapping-based) v0.13.1

[ program ] => salmon

[ command ] => quant

[ threads ] => { 5 }

[ index ] => { ~/CellNet/CellNetLocal/ref/salmon.index.mouse.052617.tgz }

[ libType ] => { U }

[ unmatedReads ] => { subset_SRR2070946_trimmed.fq }

[ output ] => { salmonRes_SRR2070946 }

Logs will be written to salmonRes_SRR2070946/logs [2024-02-28 02:07:19.419] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2024-02-28 02:07:19.419] [jointLog] [warning]

NOTE: It appears you are running salmon without the --validateMappings option. Mapping validation can generally improve both the sensitivity and specificity of mapping, with only a moderate increase in use of computational resources. Mapping validation is planned to become a default option (i.e. turned on by default) in the next release of salmon. Unless there is a specific reason to do this (e.g. testing on clean simulated data), --validateMappings is generally recommended.

[2024-02-28 02:07:19.419] [jointLog] [info] parsing read library format [2024-02-28 02:07:19.419] [jointLog] [info] There is 1 library. Exception : [Error: The index version file ~/CellNet/CellNetLocal/ref/salmon.index.mouse.052617.tgz/versionInfo.json doesn't seem to exist. Please try re-building the salmon index.] ~/anaconda3/envs/salmon/bin/salmon quant was invoked improperly. For usage information, try ~/anaconda3/envs/salmon/bin/salmon quant --help Exiting. ./salmonRes_SRR2070926/quant.sf Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") : cannot open file './salmonRes_SRR2070926/quant.sf': No such file or directory

I can see versionInfo.json in the salmon.index.mouse.052617.tgz. I am not sure why it can not see it.