Logs will be written to salmonRes_SRR2070946/logs
[2024-02-28 02:07:19.419] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
[2024-02-28 02:07:19.419] [jointLog] [warning]
NOTE: It appears you are running salmon without the --validateMappings option.
Mapping validation can generally improve both the sensitivity and specificity of mapping,
with only a moderate increase in use of computational resources.
Mapping validation is planned to become a default option (i.e. turned on by default) in
the next release of salmon.
Unless there is a specific reason to do this (e.g. testing on clean simulated data),
--validateMappings is generally recommended.
[2024-02-28 02:07:19.419] [jointLog] [info] parsing read library format
[2024-02-28 02:07:19.419] [jointLog] [info] There is 1 library.
Exception : [Error: The index version file ~/CellNet/CellNetLocal/ref/salmon.index.mouse.052617.tgz/versionInfo.json doesn't seem to exist. Please try re-building the salmon index.]
~/anaconda3/envs/salmon/bin/salmon quant was invoked improperly.
For usage information, try ~/anaconda3/envs/salmon/bin/salmon quant --help
Exiting.
./salmonRes_SRR2070926/quant.sf
Error in file(file, "rt") : cannot open the connection
In addition: Warning message:
In file(file, "rt") :
cannot open file './salmonRes_SRR2070926/quant.sf': No such file or directory
I can see versionInfo.json in the salmon.index.mouse.052617.tgz. I am not sure why it can not see it.
I am running salmon and I am getting this error.
salmon (mapping-based) v0.13.1
[ program ] => salmon
[ command ] => quant
[ threads ] => { 5 }
[ index ] => { ~/CellNet/CellNetLocal/ref/salmon.index.mouse.052617.tgz }
[ libType ] => { U }
[ unmatedReads ] => { subset_SRR2070946_trimmed.fq }
[ output ] => { salmonRes_SRR2070946 }
Logs will be written to salmonRes_SRR2070946/logs [2024-02-28 02:07:19.419] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored. [2024-02-28 02:07:19.419] [jointLog] [warning]
NOTE: It appears you are running salmon without the
--validateMappings
option. Mapping validation can generally improve both the sensitivity and specificity of mapping, with only a moderate increase in use of computational resources. Mapping validation is planned to become a default option (i.e. turned on by default) in the next release of salmon. Unless there is a specific reason to do this (e.g. testing on clean simulated data),--validateMappings
is generally recommended.[2024-02-28 02:07:19.419] [jointLog] [info] parsing read library format [2024-02-28 02:07:19.419] [jointLog] [info] There is 1 library. Exception : [Error: The index version file ~/CellNet/CellNetLocal/ref/salmon.index.mouse.052617.tgz/versionInfo.json doesn't seem to exist. Please try re-building the salmon index.] ~/anaconda3/envs/salmon/bin/salmon quant was invoked improperly. For usage information, try ~/anaconda3/envs/salmon/bin/salmon quant --help Exiting. ./salmonRes_SRR2070926/quant.sf Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") : cannot open file './salmonRes_SRR2070926/quant.sf': No such file or directory
I can see versionInfo.json in the salmon.index.mouse.052617.tgz. I am not sure why it can not see it.