🐟 🍣 🍱 Highly-accurate & wicked fast transcript-level quantification from RNA-seq reads using selective alignment
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error when trying to follow tutorial #931
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mocherry closed 6 months ago
Dear all,
I started to follow the Salmon "Real data example" on https://combine-lab.github.io/salmon-tutorials/2021/ont-long-read-quantification/
Everything goes fine up to: minimap2 –t 8 –a -x map-ont –p 1.0 –N 100 minimap2_spike_index SRR6058582.fastq
when I get the following error: [ERROR] failed to open file '–t': No such file or directory
???
Help would be appreciated. Thanks and best, Matthias