CSB5 / lofreq

LoFreq Star: Sensitive variant calling from sequencing data
http://csb5.github.io/lofreq/
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Filter depend on number of cores? #131

Open bernt-matthias opened 2 years ago

bernt-matthias commented 2 years ago

I run lofreq call-parallel with different numbers for --pp-threads and get different vcf output:

lofreq call-parallel --pp-threads 1 --verbose --ref 'reference.fa' --out variants.vcf --min-cov 1 --max-depth 1000000 --min-bq 6 --min-alt-bq 6 --min-mq 0 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.01 --bonf dynamic reads.bam

##FILTER=<ID=min_snvqual_33,Description="Minimum SNV Quality (Phred) 33">
##FILTER=<ID=min_snvqual_38,Description="Minimum SNV Quality (Phred) 38">

lofreq call-parallel --pp-threads 2 --verbose --ref 'reference.fa' --out variants.vcf --min-cov 1 --max-depth 1000000 --min-bq 6 --min-alt-bq 6 --min-mq 0 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.01 --bonf dynamic reads.bam

##FILTER=<ID=min_snvqual_38,Description="Minimum SNV Quality (Phred) 38">
##FILTER=<ID=min_snvqual_38,Description="Minimum SNV Quality (Phred) 38">

Wondering if this is expected/desired.