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CahanLab / singleCellNet

SingleCellNet: classify single cells across species and platforms
MIT License
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Transcription factor #55

Closed bali-eng closed 5 months ago

bali-eng commented 5 months ago

Hi,

Thank you for your excellent work. In Figure 2 (E), you mentioned that in this classification heatmap, the columns annotate the five different transcription factor pairs used to generate each iN profile. My question is, how did you define these five transcription factor pairs? Could you please elaborate on that?

Thanks

pcahan1 commented 5 months ago

The columns that are referred to in the caption of Fig 2E of PMID: 31377170 are the broad groups of columns indicated by the color band at the top of the heatmap. Each group of columns or cells, was converted to an iN with a different set of TFs, as indicated by the legend, for example the first group of cells was induced with Ascl2 and Nr4a2. These pairs of TFs were identified and experimentally tested as described in PMID: 29743677. Thank you.

bali-eng commented 5 months ago

Thanks for your clarification.