Open JackCurragh opened 9 months ago
Yeah I think the module plan is very good, thank you for doing it. We could also integrate subsampling at the start of the pipeline. Ed had proposed this for my dissertation, but I couldn't do it it due to time constraints. I have now added FASTP to our pipeline and am in the process of implementing subworkflows. Once I get it working, I will update everything on github.
Description of feature
Here I attempt to break down the required/desired modules for each processing stage of the workflow:
Pre-Processing
[X] Quality Control (FastQC)
[ ] FASTP (Adapter Removal)
[X] UMI-tools (UMI handling)
Alignment
[ ] Bowtie (Transcriptome Alignment)
Translated ORF Detection
Quantification
Differential Expression
Please reply here with any other packages we should consider!