taxa_abund =null

[Elixir0822]

Hello，chi I want to trans the phyloseq format to microeco and do Lefse analysis，but the taxa_abund is null

dataset <- phyloseq2meco(ps) dataset microtable-class object: sample_table have 15 rows and 1 columns otu_table have 274 rows and 15 columns tax_table have 274 rows and 7 columns phylo_tree have 274 tips rep_fasta have 274 sequences dataset$taxa_abund NULL lefse <- trans_diff$new(dataset = dataset,

• method = "lefse",
• group = "Group",
• alpha = 0.01,
• lefse_subgroup = NULL,
• p.adjust.methods=) No taxa_abund list found. Calculate it with cal_abund function ... The result is stored in object$taxa_abund ... 489 input features ... 442 features are remained after removing unknown features ... Start Kruskal-Wallis rank sum test for Group ... 12 taxa found significant ... After P value adjustment, 0 taxa found significant ... Error in private$check_taxa_number(sel_taxa, p_adjust_method) : No significant feature found! To disable p value adjustment, please use p_adjust_method = "none"!

[ChiLiubio]

Hi. The message shows there is no significant feature. Please use alpha = 0.05 and try again. Donot worry abunt taxa_abund. The function can automatically calculate and get taxa_abund when it is NULL.

[Elixir0822]

Thank you so much！