amandaraud
commented
1 year ago During a meeting with IL, AZ and ARW 2023-05-11, it was decided that this should be part of a bigger project, to include the dilution in the prep instead of sequencing. It would be preferable if the normalization could be performed by a robot, for example when preparing the qPCR plate. As we are planning to move the qPCR setup from Bravo to Hamilton, we will investigate if it is possible to add a normalization step in the Hamilton protocol. When we have decided on how to include the normlaization in the prep, we will continue on this issue and update the WGS workflow.
Karl-Svard
Lims Issue
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Work Flow: WGS PCR free v5 Protocol: NovaSeq v3 Step: N/A
Description: Because metagenome samples are sequenced less deep than other WGS samples, they are regularly diluted 1:10 by sequencing staff in order to be able to pipette more accurately when pooling. The dilution is manually calculated and the new concentration is entered into Lims in the Define run step. It would be good if the dilution was calculated by Lims.