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Clinical-Genomics / clinical_EPPs

EPPs used in clinical genomics LIMS
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Add index check when pooling for hyb #205

Closed cesve closed 3 years ago

cesve commented 3 years ago

Lims Issue

Work Flow: TWIST v3 Protocol: Target Enrichment TWIST v2 Step: Pool samples for hybridization TWIST

Description: We would like to add an index check when making pools for hyb to make sure that samples don't have the same index.

cesve commented 3 years ago

@mayabrandi we have a script for index check in the sequencing protocol. Can I just add that button to step Pool samples for hybridization TWIST?

cesve commented 3 years ago

@mayabrandi do you have time to check if it's just to add the button to the step? See comment above. Thanks!

cesve commented 3 years ago

The button "Check Duplicate Index" was added to the step. When testing the script without any index clashes the script worked well. A test where the same index well but different sets of indicies (set A/ set C) was tried, this didn't give any warnings which is good.

index check inga krockar

When a test was set up using the same index for some samples, it was not possible to pool the samples. A warning appeared saying it's not possible to pool samples with the same index.

IMG_9913

cesve commented 3 years ago

The function that made it impossible to add libraries with the same index to the same pool looks at the name of the index while the script in the step looks at the sequence of the index.