Add reverse complementing index 2 in samplesheet based on Novaseq Control Software version and reagent kit version

barrystokman commented 3 years ago

This PR adds constructing a novaseq samplesheet based on new run parameters: Novaseq Control Software version and reagent kit version

This PR solves issue https://github.com/Clinical-Genomics/demultiplexing/issues/111

How to prepare for test:

[x] ssh to thalamus
[x] Generate current prod samplesheet of HGJJKDSXY for comparison: demux sheet fetch -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/
[x] install on stage: bash servers/resources/clinical-preproc.scilifelab.se/update-demux-stage.sh feat/reverse-complement-index bash servers/resources/clinical-preproc.scilifelab.se/update-servers-stage.sh feat/demux-novaseq-dirs
[x] set proper alias (yes, really): alias demux-stage='$HOME/miniconda2/envs/stage/bin/demux --config /home/hiseq.clinical/STAGE/servers/config/clinical-preproc.scilifelab.se/demux-stage.yaml'

How to test:

Preconditions:

[x] prepare run parameter files in /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/
[x] cp <run parameters file> RunParameters.xml before running each test
[x] Note: CLI command ran in automation: demux sheet fetch -a nova -p -L <flowcell_id>
[x] Generate current prod samplesheet of HGJJKDSXY for comparison: demux-stage sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/HGJJKDSXY_prod_samplesheet.csv

Test Cases:

[x] TC1: old software control version: cp RunParameters_oldSCV.xml RunParameters.xml: demux-stage sheet fetch -a nova -p -L HGJJKDSXY > TC1_samplesheet.csv
[x] TC2: new software control version, old kit: cp RunParameters_newSCV_oldkit.xml RunParameters.xml: demux-stage sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC2_samplesheet.csv
[x] TC3: new software control version, new kit: cp RunParameters_newSCV_newkit.xml RunParameters.xml: demux-stage sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC3_samplesheet.csv
[x] TC4: fluffy: cp RunParameters_fluffy.xml RunParameters.xml: demux-stage sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC4_samplesheet.csv
[x] TC5: regression: demux-stage sheet fetch -a nova -L HGJJKDSXY
[x] TC6: regression: demux-stage sheet fetch -a nova -p HGJJKDSXY
[x] TC7: regression: demux-stage sheet fetch -a nova HGJJKDSXY

Expected test outcome:

[x] TC1: constructed samplesheet equal to current result of cli command: indexes padded to length 10, index1 ends with AT, index2 ends with AC, samplesheet is equal to HGJJKDSXY_prod_samplesheet.csv
[x] TC2: constructed samplesheet equal to current result of cli command: indexes padded to length 10, samplesheet is equal to HGJJKDSXY_prod_samplesheet.csv
[x] TC3: indexes padded to length 10, index 2 reverse complemented
[x] TC4: constructed samplesheet equal to current result of cli command: index 2 reverse complemented
[x] TC5: runs without errors
[x] TC6: warning printed to terminal, aborted
[x] TC7: runs without errors, raw unmodified samplesheet

Review:

[ ] code approved by
[x] tests executed by @barrystokman
[ ] "Merge and deploy" approved by Thanks for filling in who performed the code review and the test!

This version is a:

[ ] MAJOR - when you make incompatible API changes
[x] MINOR - when you add functionality in a backwards compatible manner
[ ] PATCH - when you make backwards compatible bug fixes or documentation/instructions

barrystokman commented 3 years ago

TC1:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC1_samplesheet.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC1_samplesheet.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,TTCAGGTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,TTGGTGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,CGCGGTTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,GACGAGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,TATAACCTAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,AGACTTGGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,CTTAAGCCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,TCCGGATTAC,300186,N,R1,script,300186
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff HGJJKDSXY_prod_samplesheet.csv TC1_samplesheet.csv

:heavy_check_mark:

barrystokman commented 3 years ago

TC2:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC2_samplesheet.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC2_samplesheet.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,TTCAGGTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,TTGGTGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,CGCGGTTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,GACGAGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,TATAACCTAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,AGACTTGGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,CTTAAGCCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,TCCGGATTAC,300186,N,R1,script,300186
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff HGJJKDSXY_prod_samplesheet.csv TC2_samplesheet.csv

:heavy_check_mark:

barrystokman commented 3 years ago

TC3:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC3_samplesheet.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC3_samplesheet.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,GACCTGAAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,CTCACCAAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,GAACCGCGGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,CTCTCGTCGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,AGGTTATAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,CCAAGTCTGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,GGCTTAAGGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,AATCCGGAGT,300186,N,R1,script,300186

:heavy_check_mark:

barrystokman commented 3 years ago

TC4:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -p -L HGJJKDSXY > /home/hiseq.clinical/STAGE/novaseq/runs/201111_A00621_0305_BHGJJKDSXY/TC4_samplesheet.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC4_samplesheet.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,GACCTGAA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,CTCACCAA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,GAACCGCG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,CTCTCGTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,AGGTTATA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,CCAAGTCT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,GGCTTAAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,AATCCGGA,300186,N,R1,script,300186

:heavy_check_mark:

barrystokman commented 3 years ago

TC5:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -L HGJJKDSXY                                                                                                                                                        
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,TTCAGGTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,TTGGTGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,CGCGGTTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,GACGAGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,TATAACCT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,AGACTTGG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,CTTAAGCC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,TCCGGATT,300186,N,R1,script,300186

:heavy_check_mark:

barrystokman commented 3 years ago

TC6:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova -p HGJJKDSXY
Please specify an index length when using the pad option! Use --longest or --indexlength.
Aborted!

:heavy_check_mark:

barrystokman commented 3 years ago

TC7:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux sheet fetch -a nova HGJJKDSXY                                                                                                                                                           
[Data]                                                                                                                                                                                                                                                        
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project                                                                                                                                                                          
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,TTCAGGTC,300186,N,R1,script,300186                                                                                                                                                                                        
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,TTGGTGAG,300186,N,R1,script,300186                                                                                                                                                                                       
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,CGCGGTTC,300186,N,R1,script,300186                                                                                                                                                                                       
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,GACGAGAG,300186,N,R1,script,300186                                                                                                                                                                                        
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,TATAACCT,300186,N,R1,script,300186                                                                                                                                                                                       
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,AGACTTGG,300186,N,R1,script,300186                                                                                                                                                                                        
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,CTTAAGCC,300186,N,R1,script,300186                                                                                                                                                                                       
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,TCCGGATT,300186,N,R1,script,300186

:heavy_check_mark:

barrystokman commented 3 years ago

To be deployed together with https://github.com/Clinical-Genomics/servers/pull/461

barrystokman commented 3 years ago

Tests and test fixtures are WIP

barrystokman commented 3 years ago

RETESTS:

TC1:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -p -L HGJJKDSXY > TC1_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC1_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,TTCAGGTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,TTGGTGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,CGCGGTTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,GACGAGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,TATAACCTAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,AGACTTGGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,CTTAAGCCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,TCCGGATTAC,300186,N,R1,script,300186
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff TC1_samplesheet.csv TC1_samplesheet_RETEST.csv

:heavy_check_mark:

TC2:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -p -L HGJJKDSXY > TC2_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC2_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,TTCAGGTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,TTGGTGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,CGCGGTTCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,GACGAGAGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,TATAACCTAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,AGACTTGGAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,CTTAAGCCAC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,TCCGGATTAC,300186,N,R1,script,300186
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff TC2_samplesheet.csv TC2_samplesheet_RETEST.csv

:heavy_check_mark:

TC3:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -p -L HGJJKDSXY > TC3_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC3_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAAAT,GACCTGAAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAAAT,CTCACCAAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACTAT,GAACCGCGGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGCAT,CTCTCGTCGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTCAT,AGGTTATAGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTGAT,CCAAGTCTGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGTAT,GGCTTAAGGT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAACAT,AATCCGGAGT,300186,N,R1,script,300186
You have mail in /var/spool/mail/hiseq.clinical
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff TC3_samplesheet.csv TC3_samplesheet_RETEST.csv

:heavy_check_mark:

TC4:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -p -L HGJJKDSXY > TC4_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC4_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,GACCTGAA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,CTCACCAA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,GAACCGCG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,CTCTCGTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,AGGTTATA,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,CCAAGTCT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,GGCTTAAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,AATCCGGA,300186,N,R1,script,300186
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ diff TC4_samplesheet.csv TC4_samplesheet_RETEST.csv

TC5:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -L HGJJKDSXY > TC5_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC5_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,TTCAGGTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,TTGGTGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,CGCGGTTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,GACGAGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,TATAACCT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,AGACTTGG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,CTTAAGCC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,TCCGGATT,300186,N,R1,script,300186

:heavy_check_mark:

TC6:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova -p HGJJKDSXY
Please specify an index length when using the pad option! Use --longest or --index_length.
Aborted!

:heavy_check_mark:

TC7:

(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ demux-stage sheet fetch -a nova HGJJKDSXY > TC7_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC7_samplesheet_RETEST.csv
(stage)[hiseq.clinical@thalamus 201111_A00621_0305_BHGJJKDSXY]$ less TC7_samplesheet_RETEST.csv | head
[Data]
FCID,Lane,SampleID,SampleRef,index,index2,SampleName,Control,Recipe,Operator,Project
HGJJKDSXY,1,ACC7198A3,hg19,CGTTAGAA,TTCAGGTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A16,hg19,GACCTGAA,TTGGTGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A18,hg19,TCTCTACT,CGCGGTTC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A7,hg19,GATTCTGC,GACGAGAG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A20,hg19,CTCTCGTC,TATAACCT,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A9,hg19,TCGTAGTG,AGACTTGG,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A13,hg19,TAAGTGGT,CTTAAGCC,300186,N,R1,script,300186
HGJJKDSXY,1,ACC7198A15,hg19,CGGACAAC,TCCGGATT,300186,N,R1,script,300186

:heavy_check_mark:

barrystokman commented 3 years ago

Bumped:

Deployed on thalamus:

Clinical-Genomics / demultiplexing