As a bioinfo prod member i want the iSeq sample sheet to be in the correct format so that I don't have to do a manual intervention.
Problem: The current demux sheet fetch -a iseq -p --longest <FC ID> returns index2 in the incorrect format for direct use in demultiplexing. It needs to be reverse complement from how it is today. The reverse complement has to be done on the padded sequence.
Suggest solution:
In the existing code add a reverse complement step after padding of index2 (only).
update document 1161 demultiplexing
Test case on thalamus:
/home/hiseq.clinical/iSeq/runs/20190528_FS10000534_7_BPC29611-3324
FC ID = BPC29611-3324
ACC5726A1 current index2 = ACCTTAGTGT, should be = ACACTAAGGT
As a bioinfo prod member i want the iSeq sample sheet to be in the correct format so that I don't have to do a manual intervention.
Problem: The current
demux sheet fetch -a iseq -p --longest <FC ID>returns index2 in the incorrect format for direct use in demultiplexing. It needs to be reverse complement from how it is today. The reverse complement has to be done on the padded sequence.Suggest solution:
Test case on thalamus:
/home/hiseq.clinical/iSeq/runs/20190528_FS10000534_7_BPC29611-3324FC ID = BPC29611-3324 ACC5726A1 current index2 =ACCTTAGTGT, should be =ACACTAAGGTDoD: